| 包装 | 价格(元) |
| 1g | 电议 |
| 5g | 电议 |
Cell lines | Sheep skin fibroblasts, SSFs |
Preparation Method | The SSFs cultured in vitro were treated with different concentrations of single antibiotic Geneticin, G-418 Sulfate. After 12 days of treatment, the SSFs were digested, and the number of dead and alive cells was counted after placental blue staining to determine the lowest lethal dose of Geneticin, G-418 Sulfate leading to the death of all SSFs. |
Reaction Conditions | 100-200ug/ml Geneticin, G-418 Sulfate for 1 day |
Applications | Treatment with 100 μg/mL Geneticin, G-418 Sulfate for 12 days did not lead to the death of all SSFs, but still (44.7 0.05)% of SSFs survived.Continuous treatment with 200 μg/mL Geneticin, G-418 Sulfate for 12 days can lead to the death of all SSFs. Therefore, the minimum lethal concentration of Geneticin, G-418 Sulfate leading to the death of SSFs is 200 μg/mL. |
| 文献引用 | |
| 产品描述 | Geneticin, G-418 Sulfate is a class of aminoglycoside antibiotics, whose structure is similar to neomycin kanamycin. It can interfere with the function of 80S ribosomes in eukaryotic cells, thereby blocking protein synthesis and leading to eukaryotic cell death[1,4]. Geneticin, G-418 Sulfate from 1 to 300 g/ mL is often used for clonal selection in prokaryotes and eukaryotes. Antibiotic resistance assay of clinically isolated bacterial strains found that all strains carrying 3' -o-aminoglycoside phosphotransferases were tolerant to Geneticin, G-418 Sulfate[2]. Treatment with 100 μg/mL Geneticin, G-418 Sulfate for 12 days did not lead to the death of all SSFs, but still (44.7 0.05)% of SSFs survived. Continuous treatment with 200 μg/mL Geneticin, G-418 Sulfate for 12 days can lead to the death of all SSFs. Therefore, the minimum lethal concentration of Geneticin, G-418 Sulfate leading to the death of SSFs is 200 μg/mL[5]. Geneticin, G-418 Sulfate has antiviral activity against bovine viral diarrhea virus (BVDV). Geneticin, G-418 Sulfate can prevent cytopaplasia effect (CPE) caused by DENV-2 infection of BHK cells in a dose-dependent manner, with an EC50 value of 3 0.4 g/ml[3]. In vivo, Geneticin, G-418 Sulfate destabilizes mRNAs broadly, in that the majority of mRNAs in mESCs have reduced stability when mESCs are treated with Geneticin, G-418 Sulfate. The mRNAs with half-lives that are most reduced by treatment with Geneticin, G-418 Sulfate are enriched for select optimal codons, containing G/C at the wobble position[7].The expression of antioxidant stress kinase-related genes and apoptotic genes in donor cells treated with different concentrations of Geneticin, G-418 Sulfate significantly changed, but the DNA methylation level did not change.The in vitro development efficiency of nuclear transfer embryos from Geneticin, G-418 Sulfate-treated donor cells was significantly lower than that of controls[6]. References: |
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