Animal experiment:

Mice: To test the in vivo efficacy of PF-429242 in regulating SREBP target genes, male CD1 mice are dosed i.p. with 10 or 30 mg/kg PF-429242 or saline once every 6 over a 24-h period. Mice are euthanized 6 h after the final dose, and liver tissue is collected, frozen rapidly in liquid nitrogen, and stored at -80℃. For RNA isolation, 50 to 100 mg of frozen liver tissue from each sample is homogenized in 1 ml of TRIzol reagent. Total RNA is extracted following the manufacturer's instructions, and the resulting total RNA from each sample underwent DNA-free treatment[1].