Apamin TFA

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Apamin TFA

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
500 μg	电议
1mg	电议
5mg	电议
10mg	电议

产品名称

Apamine TFA

产品介绍

Apamin TFA (Apamine TFA) 是在蜂毒素中发现的一种 18 个氨基酸的肽类神经毒素，是一种Ca²⁺激活的K⁺(SK) 通道的特异性和选择性的阻断剂，具有抗炎和抗纤维化的作用。

生物活性

Apamin TFA (Apamine TFA) is an 18 amino acid peptide neurotoxin found in apitoxin (bee venom), is known as a specifically selective blocker ofCa²⁺-activatedK⁺(SK) channels and exhibits anti-inflammatory and anti-fibrotic activity^[1].

IC₅₀& Target

K⁺channel^[1]

体外研究
(In Vitro)

Apamin (0.5-2 μg/mL; 24 hours; HSC-T6 cells) treatment markedly reduces the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Apamin treatment abrogats the activation of p-Smad2/3 and Smad4 induced by TGF-β1^[1].

Western Blot Analysis^[1]

Cell Line:	HSC-T6 cells
Concentration:	0.5 μg/mL, 1 μg/mL and 2 μg/mL
Incubation Time:	24 hours
Result:	Markedly reduced the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Abrogated the activation of p-Smad2/3 and Smad4 induced by TGF-β1.

体内研究
(In Vivo)

Apamin (0.1 mg/kg; intraperitoneal injection; twice a week; for 4 weeks; C57BL/6 male mice) treatment results in decreased liver injury and proinflammatory cytokine levels. Apamin suppresses the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-fed mice^[1].

Animal Model:	8-week-old C57BL/6 male mice (20-25 g) with DDC feeding^[1]
Dosage:	0.1 mg/kg
Administration:	Intraperitoneal injection; twice a week; for 4 weeks
Result:	Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.

分子量

2141.36

性状

Solid

Formula

C₈₁H₁₃₂F₃N₃₁O₂₆S₄

Sequence

Cys-Asn-Cys-Lys-Ala-Pro-Glu-Thr-Ala-Leu-Cys-Ala-Arg-Arg-Cys-Gln-Gln-His-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)

Sequence Shortening

CNCKAPETALCARRCQQH-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 50 mg/mL(23.35 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.4670 mL	2.3350 mL	4.6699 mL
5 mM	0.0934 mL	0.4670 mL	0.9340 mL
10 mM	0.0467 mL	0.2335 mL	0.4670 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (46.70 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。