X-Gluc cyclohexanamine is a dye reagent for the detection ofβ-glucuronidase(GUS), an enzyme produced byE. coli. X-Gluc cyclohexanamine can be used to detectE. colicontamination in food, water and the urinary tract (GUSas a specific detection indicator). X-Gluc cyclohexanamine is also widely used inmolecular biologyexperiments to label and detect the expression of target genes (reacts with theGUS gene, appears blue)^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35℃ and observe 16-24 h after inoculation.

521.79

Solid

C₂₀H₂₆BrClN₂O₇

114162-64-0

5-溴-4-氯-3-吲哚葡萄糖苷

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)