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X-Gluc cyclohexanamine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
X-Gluc cyclohexanamine图片
CAS NO:114162-64-0
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
5-溴-4-氯-3-吲哚葡萄糖苷
产品介绍
X-Gluc cyclohexanamine 是一种检测β-葡萄糖醛酸酶(GUS) 的染料试剂 (与GUS基因反应,显现蓝色)。β-葡萄糖醛酸酶是一种由大肠杆菌产生的酶,可作为食物、水和泌尿道中大肠杆菌污染的特异性检测指标。X-Gluc cyclohexanamine 也可广泛地用于分子生物学实验中,标记和检测目的基因的表达 (GUS报告基因)。
生物活性

X-Gluc cyclohexanamine is a dye reagent for the detection ofβ-glucuronidase(GUS), an enzyme produced byE. coli. X-Gluc cyclohexanamine can be used to detectE. colicontamination in food, water and the urinary tract (GUSas a specific detection indicator). X-Gluc cyclohexanamine is also widely used inmolecular biologyexperiments to label and detect the expression of target genes (reacts with theGUS gene, appears blue)[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35℃ and observe 16-24 h after inoculation.

分子量

521.79

性状

Solid

Formula

C20H26BrClN2O7

CAS 号

114162-64-0

中文名称

5-溴-4-氯-3-吲哚葡萄糖苷

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)