Phoyunnanin E，分离自铁皮石斛，具有抗迁移活性。Phoyunnanin E可用于癌症研究。
| 生物活性 | Phoyunnanin E, isolated from Dendrobium venustum, possesses anti-migration activity. Phoyunnanin E can be used for the research ofcancer[1]. |
体外研究
(In Vitro) | Phoyunnanin E (0~10 μM; 48 hours; H460 cells) decreases cell migration through integrin downregulation[1].
Phoyunnanin E (0~100 μM; 24 and 48 hours; H460 cells) shows that the proliferation rate of treated cells have significantly decreased in a dose-dependent manner at the concentration of 10 μM at 48 hours[1].
Phoyunnanin E (0~100 μM; 24 hours; H460 cells) induces cytotoxic effects at the concentrations from 50 to 100 μM and induces a significant increase in apoptotic and necrotic cells at 20~100 μM[1].
Phoyunnanin E (0~10 μM; 48 hours; H460, H292 and A549 cells) significantly decreases the number of cells invading across the matrix and transwell filter within 24 hours in a dose-dependent manner[1].
Phoyunnanin E attenuates anchorage-independent growth and migration of lung cancer H460 cells. Phoyunnanin E significantly inhibits cell migration across the wound space at the concentrations of 5 and 10 μM, at 24 h and 48 h, compared to the non-treated control. Phoyunnanin E (0~10 μM; 48 hours) exhibits a significant decrease in filopodia numbers at the protrusion edges of the cells in a dose-dependent manner. Phoyunnanin E decreases cancer cell migration in other human lung cancer cells. Phoyunnanin E decreases cancer cell invasion. Phoyunnanin E suppresses epithelial-mesenchymal transition (EMT). Phoyunnanin E down-regulates integrins αv and β3 and the regulatory proteins in cell migration[1].
Western Blot Analysis[1] | Cell Line: | H460 cells | | Concentration: | 1~10 μM | | Incubation Time: | 48 hours | | Result: | Decreased cell migration through integrin downregulation. |
Cell Proliferation Assay[1] | Cell Line: | H460 cells | | Concentration: | 0~100 μM | | Incubation Time: | 24 and 48 hours | | Result: | The proliferation rate of treated cells had significantly decreased in a dose-dependent manner at the concentration of 10 μM at 48 h. |
Apoptosis Analysis[1] | Cell Line: | H460 cells | | Concentration: | 0~100 μM | | Incubation Time: | 24 hours | | Result: | Induced a significant increase in apoptotic and necrotic cells at 20~100 μM. |
Cell Cytotoxicity Assay[1] | Cell Line: | H460 cells | | Concentration: | 0~100 μM | | Incubation Time: | 24 hours | | Result: | Induced cytotoxic effects at the concentrations from 50 to 100 μM. |
Immunofluorescence[1] | Cell Line: | H460, H292 and A549 cells | | Concentration: | 0~10 μM | | Incubation Time: | 48 hours | | Result: | Significantly decreased the number of cells invading across the matrix and transwell filter within 24 hours in a dose-dependent manner. |
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| 来源 | - Plants
- Orchidaceae
- Dendrobium nobileLindl.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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