Traumatic Acid 是一种伤口愈合剂，也是细胞分裂素 (植物激素)。Traumatic Acid 能增强培养的人类皮肤成纤维细胞的胶原蛋白的生物合成。Traumatic Acid 可抑制 MCF-7 乳腺癌细胞的存活率并增强细胞凋亡和氧化应激。Traumatic Acid 可用于癌症、血液循环系统疾病 (包括动脉高血压)，以及与氧化应激和胶原蛋白生物合成障碍有关的皮肤病的研究。
| 生物活性 | Traumatic Acid is a wound healing agent and a cytokinin (phytohormone). Traumatic Acid enhances the biosynthesis of collagen in cultured human skin fibroblasts. Traumatic Acid inhibits MCF-7 breastcancercells viability and enhancesapoptosisand oxidative stress. Traumatic Acid can be used in studies ofcancer, circulatory disorders (including arterial hypertension), and skin diseases associated with oxidative stress and impaired collagen biosynthesis[1][2]. |
| IC50& Target | IC50: collagen biosynthesis[1] |
体外研究
(In Vitro) | Traumatic Acid (0.1, 1 μM; 5 days) significantly increases cell number in fibroblasts[1].
Traumatic Acid (0.1, 1 μM; 5 days) increases content of GPX activity and reduced glutathione, as well as decreases membrane phospholipid peroxidation in fibroblasts[1].
Traumatic Acid (0.1, 1 μM; 5 days) enhances the production and secretion of medium collagen in medium of fibroblasts[1].
Traumatic Acid (100, 200, 400, 600 μM; 48 h) significantly decreases live cell number, especially after 48h treatment at 100μM and 200μM in MCF-7 cells[2].
Traumatic Acid (50-600 μM; 24, 48 h) causes dose-and time-dependent reduction in cell viability and induces apoptosis in MCF-7 cells[2].
Traumatic Acid (50-200 μM; 24, 48 h) results in an oxidative damage of protein in MCF-7 cells[2].
Traumatic Acid (100, 200 μM; 24, 48 h) efficiently enhances oxidative stress level in MCF-7 cells[2].
Cell Proliferation Assay[1] | Cell Line: | Fibroblasts | | Concentration: | 0.1, 1 μM | | Incubation Time: | 5 days | | Result: | Caused a significant increase in cell number, especially on day 1 at a concentration of 1 μM.
Increased cell number of 133 % and 118 % compared to the untreated control cells for concentrations of 1 and 0.1 μM, respectively. |
Cell Viability Assay[1] | Cell Line: | Fibroblasts | | Concentration: | 0.1, 1 μM | | Incubation Time: | 5 days | | Result: | Increased total protein content of 183 % and 90% compared to the control at concentrations of 1 and 0.1 μM on day 1.
Increased collagen content of 72 % at 0.1 μM (on the day 3) and of 51 % at 1 μM (on the day 1) compared to the control.
Increased GPX activity by 111 % and 97 % at concentrations of 1 and 0.1 μM compared to the control.
Increased content of reduced glutathione of 86 % and 80% at 0.1 and 1 μM, respectively.
Decreased membrane phospholipid peroxidation. |
Cell Viability Assay[2] | Cell Line: | MCF-7 cells | | Concentration: | 100, 200, 400, 600 μM | | Incubation Time: | 48 h | | Result: | Decreasd live cell number of about 76% at 100 μM concentration. |
Cell Viability Assay[2] | Cell Line: | MCF-7 cells | | Concentration: | 50-200 μM | | Incubation Time: | 24, 48 h | | Result: | Increased thiol group content of 167% at 100μM and 24 h. |
Cell Viability Assay[2] | Cell Line: | MCF-7 cells | | Concentration: | 100, 200 μM | | Incubation Time: | 24, 48 h | | Result: | Increased the amount of ROS. |
Apoptosis Analysis[2] | Cell Line: | MCF-7 cells | | Concentration: | 50-600 μM | | Incubation Time: | 24, 48 h | | Result: | Increased level of apoptosis in a time- and dose-dependent manner. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 来源 | - Plants
- Leguminosae
- Phaseolus vulgarisLinn.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: DMSO : 250 mg/mL(1095.15 mM;Need ultrasonic) 配制储备液 | 1 mM | 4.3806 mL | 21.9029 mL | 43.8059 mL | | 5 mM | 0.8761 mL | 4.3806 mL | 8.7612 mL | | 10 mM | 0.4381 mL | 2.1903 mL | 4.3806 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (9.11 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (9.11 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (9.11 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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