Lewis X trisaccharide (Lewis X, Lex) 是一种强效 TH2 调节剂，拮抗 LPS 诱导的 IL-12 的免疫表达。Lewis X trisaccharide 是人组织血型抗原，在细胞-细胞粘附中起关键作用，是一种肿瘤标志物。Lewis X trisaccharide 也被用于血吸虫病的免疫学研究。
| 生物活性 | Lewis X trisaccharide (Lewis X, Lex) is a potent TH2 regulator, antagonizes LPS-inducedIL-12immune expression. Lewis X trisaccharide is a human histo-blood group antigen, plays an key role in cell-cell adhesion, and servers as a tumor marker. Lewis X trisaccharide is highly expressed in the outer membrane of theparasite, can be used for the immunology research of schistosomiasis[1][2][3]. |
体外研究
(In Vitro) | Lewis X trisaccharide-BSA (25 μg/mL; 30 min; before LPS stimulation of 10 ng/mL for 4 h) IL-12p40 and suppresses IL-12p70 protein expression induced byLipopolysaccharide(LPS, HY-D1056)[1].
Lewis X trisaccharide (2 μM; 30 min; before LPS stimulation of 10 ng/mL for 2 h) decreases nuclear NF-κB concentration in mice bone marrow derived dendritic cells (BDDCs)[1].
Lewis X trisaccharide-BSA (25 μg/mL; 48 h) or Lewis X trisaccharide (5 μg/mL; 48 h) plus ovalbumin (OVA, 25 μg/mL) increases cytokines (IL-4, IL-13, and INF-γ) level in mice splenocytes[1].
Lewis X trisaccharide-containing glycoconjugates stimulates B cells to proliferate and to produce factors that down-regulates the TH1immune response and up-regulates the TH2 immune response[2].
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体内研究
(In Vivo) | Lewis X trisaccharide ( 5 μg, combinded with 50 μg ovalbumin; s.c.; once a week for 2 weeks) regulates IgE/TH2 responses, and selectively increases the IgE and IgG1 responses in C3H mice, independent of the LPS-TLR4 signaling[1].
| Animal Model: | Mice (BALB/c, IL-12 deficient on a BALB/c background, TLR4-defective C3H/hej, or TLR4-wild type C3H/HeOuj mice) (6-8 weeks old)[1] | | Dosage: | 5 μg, combinded with 50 μg ovalbumin | | Administration: | Subcutaneous injection; once a week for 2 weeks | | Result: | In C3H mice, coupled with BSA (LeX-BSA) and elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic TH2 cytokines when compared with those seen in BSA-sensitized mice.
In BALB/c mice, induced by ovalbumin, significantly increased levels of specific IgE, resulted IgG2a antibodies concomitant with reduced levels of serum IL-12p70.
In IL-12-deficient BALB/c mice, attenuated the downward trend of the above indicators. |
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| 结构分类 | - Saccharides
- Polysaccharides
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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