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Apamin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:24345-16-2
包装与价格:
包装价格(元)
500 μg电议
1mg电议
5mg电议
10mg电议

产品名称
Apamine
产品介绍
Apamin (Apamine) 是在蜂毒素中发现的一种 18 个氨基酸的肽类神经毒素,是一种Ca2+激活的K+(SK) 通道的特异性和选择性的阻断剂,具有抗炎和抗纤维化的作用。
生物活性

Apamin (Apamine) is an 18 amino acid peptide neurotoxin found in apitoxin (bee venom), is known as a specifically selective blocker ofCa2+-activatedK+(SK) channels and exhibits anti-inflammatory and anti-fibrotic activity[1].

IC50& Target

K+channel[1]

体外研究
(In Vitro)

Apamin (0.5-2 μg/mL; 24 hours; HSC-T6 cells) treatment markedly reduces the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Apamin treatment abrogats the activation of p-Smad2/3 and Smad4 induced by TGF-β1[1].

Western Blot Analysis[1]

Cell Line:HSC-T6 cells
Concentration:0.5 μg/mL, 1 μg/mL and 2 μg/mL
Incubation Time:24 hours
Result:Markedly reduced the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Abrogated the activation of p-Smad2/3 and Smad4 induced by TGF-β1.
体内研究
(In Vivo)

Apamin (0.1 mg/kg; intraperitoneal injection; twice a week; for 4 weeks; C57BL/6 male mice) treatment results in decreased liver injury and proinflammatory cytokine levels. Apamin suppresses the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-fed mice[1].

Animal Model:8-week-old C57BL/6 male mice (20-25 g) with DDC feeding[1]
Dosage:0.1 mg/kg
Administration:Intraperitoneal injection; twice a week; for 4 weeks
Result:Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.
分子量

2027.34

性状

Solid

Formula

C79H131N31O24S4

CAS 号

24345-16-2

Sequence

Cys-Asn-Cys-Lys-Ala-Pro-Glu-Thr-Ala-Leu-Cys-Ala-Arg-Arg-Cys-Gln-Gln-His-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)

Sequence Shortening

CNCKAPETALCARRCQQH-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)

中文名称

蜂毒明肽

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 100 mg/mL(49.33 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM0.4933 mL2.4663 mL4.9326 mL
5 mM0.0987 mL0.4933 mL0.9865 mL
10 mM0.0493 mL0.2466 mL0.4933 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。