Systemin

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Systemin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

137181-56-7

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
25mg	电议

产品名称

系统素

产品介绍

Systemin 是一种18个氨基酸的多肽，从番茄叶子中分离出来，可诱导产生超过15种防御基因。

生物活性

Systemin, an 18-amino acid polypeptide, has been isolated from tomato leaves that is a powerful inducer of over 15 defensive genes.

体外研究
(In Vitro)

Systemin is readily transported from wound sites and is considered to be the primary systemic signal.The signal transduction pathway that mediates Systemin signaling involves linolenic acid release from membranes and subsequent conversion to jasmonic acid, a potent activator of defense gene transcription. The pathway exhibits analogies to arachidonic acid/prostaglandin signaling in animals that leads to inflammatory and acute phase responses^[1]. Systemin, an 18-amino-acid peptide signal. Systemin is cleaved from the C-terminal region of a 200-amino acid precursor protein called proSystemin. Following the cloning ofproSystemingene, which represents a single locus in the tomato genome, several lines of genetic evidence indicate that Systemin acts as an upstream component of wound-induced signaling cascades leading to defense gene expression^[2].

分子量

2010.30

Formula

C₈₅H₁₄₄N₂₆O₂₈S

CAS 号

137181-56-7

Sequence

Ala-Val-Gln-Ser-Lys-Pro-Pro-Ser-Lys-Arg-Asp-Pro-Pro-Lys-Met-Gln-Thr-Asp

Sequence Shortening

AVQSKPPSKRDPPKMQTD

中文名称

系统素

结构分类

Others

来源

Plants
Solanaceae
Lycopersicon esculentumMiller

Plants
other families

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.