In vitro activity: treatment with 5-methoxyflavone at various times (3, 6, 12, 18 and 24 h) in MOLT-4 cells results in a time dependent reduction in the levels of antiapoptotic proteins cFLIP, Mcl-1 and an increase in the proapoptotic protein BAX. 5-MF induces both TRAIL-R1(DR4) and TRAIL-R2 (DR5) in a time-dependent manner.

Kinase Assay:

Cell Assay: OLT-4 or U937 or PBMC cells are treated with DMF or TMF or PMF, 5-methoxyflavone or 2'-methoxyflavone at various concentrations for 24 h, then the cell viability is determined by the MTT assay.