Licoricidin

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Licoricidin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

30508-27-1

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议

产品名称

甘草西定

产品介绍

Licoricidin (LCD) 从甘草Glycyrrhiza uralensis Fisch中分离，具有抗癌活性。Licoricidin (LCD) 通过诱导周期停滞，诱导细胞凋亡 (apoptosis) 和自噬 (autophagy)，可用于结直肠癌的研究。Licoricidin (LCD) 通过抑制肿瘤血管生成和淋巴管生成以及肿瘤组织局部微环境的变化抑制肺转移。Licoricidin (LCD) 通过体外和体内Akt和NF-κB途径的失活，增强吉西他滨诱导的骨肉瘤 (OS) 细胞的细胞毒性。Licoricidin (LCD) 通过 ROS 清除阻断 UVA 诱导的光老化，限制MMP-1的活性，被认为是新的局部应用的抗衰老制剂中的活性成分。

生物活性

Licoricidin (LCD) is isolated fromGlycyrrhiza uralensis Fisch, possesses anti-cancer activities. Licoricidin (LCD) inhibit SW480 cells (IC₅₀=7.2 μM) by inducing cycle arrest,apoptosisandautophagy, and is a potential chemopreventive or chemotherapeutic agent against colorectalcancer^[1]. Licoricidin (LCD) inhibits Lung Metastasis by inhibition of tumor angiogenesis and lymphangiogenesis as well as changes in the local microenvironment of tumor tissues the anticarcinogenic effect^[1]. Licoricidin enhanced gemcitabine-induced cytotoxicity in Osteosarcoma (OS) cells by inactivation of theAktandNF-κBpathways in vitro and in vivo^[3]. Licoricidin blocks UVA-induced photoaging via ROS scavenging, limits the activity ofMMP-1, it can be considered as an active ingredient in new topically applied anti-ageing formulations^[4].

体外研究
(In Vitro)

Licoricidin (LCD) (0-20 μM; 24 hours) dose-dependently inhibits the viability of colon cancer cell lines with various pathological and genetic characters, namely SW480, HCT116, SW620 and LoVo cells, with IC₅₀values of 7.2, 5.4, 4.5 and 5.1 μM, respectively^[1].
Licoricidin (LCD) (0-20 μM; 0-12 hours) induces cell apoptosis was accompanied with the activation of caspase-3 by cleavage in a time- and dose-dependent manner^[1].
Licoricidin (LCD) (0-20 μM; 0-12 hours) induces autophagy of SW480 cells, increases the cleavage of LC3-I to LC3-II and the degradation of p62 in a time and dose dependent manner^[1].
Licoricidin (LCD) (0-5 μg/ml; 18 hours) inhibits cell migration, MMP-9 secretion, and VCAM expression in 4T1 cells^[2].

Cell Viability Assay^[1]

Cell Line:	SW480, HCT116, SW620 and LoVo cells
Concentration:	0-20 μM
Incubation Time:	24 hours
Result:	Decreased colon cancer cell lines viability.

Western Blot Analysis^[1]

Cell Line:	SW480 cells
Concentration:	0 μM, 2.5 μM, 5 μM, 10 μM, 20 μM
Incubation Time:	0 hours, 1 hour, 3 hours, 6 hours, 12 hours
Result:	Induced cell apoptosis.

体内研究
(In Vivo)

Licoricidin (LCD) (intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 15 days) significantly inhibited the growth of SW480 xenografts in nude mice with an inhibitory rate of 43.5%^[1].
Licoricidin (LCD) (intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 32 days) reduces pulmonary metastasis and the expression of CD45, CD31, HIF-1α, iNOS, COX-2, and VEGF-A in tumor tissues, additionally, decreases protein expression of VEGF-R2, VEGF-C, VEGF-R3, and LYVE-1 in tumor tissues of licoricidin-treated mice^[2].

Animal Model:	SW480 xenografted tumor growth in nude mice^[1]
Dosage:	5, 10, or 20 mg/kg
Administration:	Intraperitoneal injection; once daily; 15 days
Result:	Decreased tumor volumes.

Animal Model:	BALB/c mouse orthotopic model^[2]
Dosage:	5, 10, or 20 mg/kg
Administration:	Intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 32 days
Result:	Inhibited Lung Metastasis of 4T1 Murine Mammary Carcinoma cells.

分子量

424.53

性状

Solid

Formula

C₂₆H₃₂O₅

CAS 号

30508-27-1

中文名称

甘草西定；甘草定；

结构分类

Phenols
Polyphenols

Others

来源

Plants
Leguminosae
Glycyrrhiza uralensisFisch.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(235.55 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.3555 mL	11.7777 mL	23.5555 mL
5 mM	0.4711 mL	2.3555 mL	4.7111 mL
10 mM	0.2356 mL	1.1778 mL	2.3555 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。