Lipoxin A4 (LXA4) 是一种内源性脂氧合酶衍生的类二十烷酸调节剂，具有强大的抗炎和促消炎双重特性。Lipoxin A4 抑制与 ERK1/2 和 NF-kB 通路相关的人表皮角质形成细胞 (NHEK) 的增殖以及炎性细胞因子/趋化因子(如IL-6，IL-8等)的产生。Lipoxin A4 抑制血清淀粉样蛋白 A (SAA) 介导的 IL-8 释放，IC50值为 25.74 nM。
| 生物活性 | Lipoxin A4 (LXA4), an endogenous lipoxygenase-derived eicosanoid mediator, has potent dual pro-resolving and anti-inflammatory properties[1]. Lipoxin A4 inhibits proliferation and inflammatory cytokine/chemokine production of human epidermal keratinocytes (NHEKs) associated with the ERK1/2 and NF-kB pathways[2]. Lipoxin A4 inhibits serum amyloid A (SAA)-mediatedIL-8release with anIC50value of 25.74 nM[3]. |
| IC50& Target | Human Endogenous Metabolite |
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体外研究
(In Vitro) | Lipoxin A4 (LXA4) inhibits the expression of IL-6 and IL-8 in NHEKs[2].
Lipoxin A4 downregulates the expression of cyclin D1[2].
Lipoxin A4 also suppresses the ERK1/2 phosphorylation and NF-kB-p65 nuclear translocation of NHEKs[2].
LXA4 (100 nM; preincubation for 30 minutes)inhibits the proliferation of NHEKs with or without stimulating by LPS (10 μg/mL)[2].
LXA4 pretreatment (100 nM for 30 minutes) downregulates the LPS-induced secretion and expression of HMGB1 in keratinocytes[1].
Cell Proliferation Assay[2] | Cell Line: | Normal human epidermal keratinocytes (NHEKs) | | Concentration: | 100 nM | | Incubation Time: | 30 minutes | | Result: | A significant increase in proliferation of NHEKs after 7 days of stimulation with LPS (10 μg/mL) was seen. However, there was a significant decrease in the proliferation of NHEKs when pretreated with LXA4 for 30 min. |
Western Blot Analysis[1] | Cell Line: | Normal human epidermal keratinocytes (NHEKs) | | Concentration: | 100 nM | | Incubation Time: | 30 minutes. | | Result: | HMGB1 protein levels in the cytoplasm of NHEKs were induced by LPS, which were decreased after preincubation with LXA4 but decreased in the nucleus after stimulation with LPS. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Pure form | -20°C | 3 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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