Isoimperatorin is a methanolic extract of the roots ofAngelica dahuricashows significant inhibitory effects on acetylcholinesterase (AChE) with theIC₅₀of 74.6 μM.

During a screening program for new agrochemicals from Chinese medicinal herbs, the ethanol extract ofNotopterygium incisumrhizomes is found to possess strong nematicidal activity against the two species of nematodes,Bursaphelenchus xylophilusandMeloidogyne incognita. Based on bioactivity-guided fractionation, the four constituents are isolated from the ethanol extract and identified as Columbianetin, Falcarindiol, Falcarinol, and Isoimperatorin. Isoimperatorin also has LC₅₀values of 21.83 μg/mL againstB. xylophilus. When using 15 min UV light treatment, falcarindiol, falcarinol, and isoimperatorin demonstrated almost five times more toxic to the southern root-knot nematodes than in dark treatment while columbianetin showed only two times more toxic. isoimperatorin has been demonstrated to possess insecticidal activity against several insects, such as the cabbage aphid (Brevicoryne brassicae)^[2]. Isoimperatorin is identified in the active fraction ofAngelica dahurica(AD) extract^[3]. Isoimperatorin is usually used as the internal standard (IS)^[4].

270.28

Solid

C₁₆H₁₄O₄

482-45-1

异欧前胡素；白芷甲素；异欧芹属素乙

• Phenylpropanoids
• Coumarins

• Plants
• Umbelliferae
• Angelica dahurica(Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. e

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Ethanol : 8.33 mg/mL(30.82 mM;Need ultrasonic)

DMSO : 2.5 mg/mL(9.25 mM;ultrasonic and warming and heat to 60℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% EtOH    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 0.83 mg/mL (3.07 mM); Clear solution

  此方案可获得 ≥ 0.83 mg/mL (3.07 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 8.3 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% EtOH    90%corn oil

  Solubility: ≥ 0.83 mg/mL (3.07 mM); Clear solution

  此方案可获得 ≥ 0.83 mg/mL (3.07 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 8.3 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中，混合均匀。