ADHP is a fluorogenic peroxidase substrate (λ_ex=530 nm, λ_em=590 nm).

To obtain the parameters K_mand k_catfor Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H₂O₂to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a K_mof 31±4 μM and the k_catof 186± 6 s^–1.The k_obsalso increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of k_obsagainst ADHP concentration K^app_onis 2.1±0.2 mM/s^[1].

257.24

Solid

C₁₄H₁₁NO₄

119171-73-2

10-乙酰基-3,7-二羟基吩嗪

Room temperature in continental US; may vary elsewhere.