您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > 4-Methylumbelliferyl phosphate disodium
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
4-Methylumbelliferyl phosphate disodium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
4-Methylumbelliferyl phosphate disodium图片
CAS NO:22919-26-2
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
4-MUP disodium
MUP disodium
产品介绍
4-Methylumbelliferyl phosphate (4-MUP) disodium 是一种阴离子有机磷酸酯,是一种酸性和碱性磷酸酶 (phosphatase) 的荧光底物。4-Methylumbelliferyl phosphate disodium 也是一种神经毒剂模拟剂。
生物活性

4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkalinephosphatasefluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant[1][2][3].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Serum acid phosphatase assay[1]:
1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.
2. Add the following agents (with the final concentration) 20 mMSodium Tartrate(HY-128476), 15 mMSodium Fluoride(HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM1, 10-Phenanthroline(HY-W004544), 6 mM8-Quinolinol(HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mMEDTA(HY-Y0682) and 6 mMEGTA(HY-D0861).
3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.
4. Mix samples thoroughly and determine fluorescence by a fluorometer.

分子量

300.11

Formula

C10H7Na2O6P

CAS 号

22919-26-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.