Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-onthiol fluorescence probefor protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells^[1]. Ex: 488 nM; Em: 540 nM.

Imaging thiols in living cells^[1]
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells^[1].
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed^[1].
The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again^[1]

391.42

Solid

C₂₂H₂₁N₃O₄

210292-65-2

Room temperature in continental US; may vary elsewhere.

DMSO : 33.33 mg/mL(85.15 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

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  Description: Naph-EA-mal is a thiol fluorescence probe, it can be used to label the protein thiols.
  Method: For measurement of total thiol level in bacteria.
  1. Incubate bacteria with Naph-EA-mal (10 min; room temperature; dark).
  2. 10⁶CFU/mL bacteria are transferred to slide for analysis.
  3. The images are captured using 100×oil immersion and observe under the confocal microscope.