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HBC514
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
HBC514图片
CAS NO:2530161-67-0
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品介绍
HBC514 本身是一种非荧光的 HBC 模拟物,但在与 Pepper RNA 适配体形成紧密复合物后可以发出强烈的绿色荧光,用于RNA标记。在活的 Pepper 标记的细胞中,HBC514-Pepper complex 可以使RNAs可视化,并且可以通过简单的洗涤和染色灵活地改变荧光。
生物活性

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

IC50& Target

IC50: RNA fluorescent dye

体外研究
(In Vitro)

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells[1].
‘Pepper’ has reduced Mg2+sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness[1].
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9)[1].

分子量

330.43

性状

Solid

Formula

C21H22N4

CAS 号

2530161-67-0

Emission (Em)
Em 495-570 nm Green
Excitation (Ex)
Ex 495-570 nm Green
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 25 mg/mL(75.66 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.0264 mL15.1318 mL30.2636 mL
5 mM0.6053 mL3.0264 mL6.0527 mL
10 mM0.3026 mL1.5132 mL3.0264 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。