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TFAX 488,SE
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
TFAX 488,SE图片
CAS NO:222164-96-7
包装:1mg
市场价:5130元

产品介绍
TFAX 488, SE 是一种绿色荧光染料,在很宽的范围内表现出 pH 不敏感性(pH 值在 4-10)。
Cas No.222164-96-7
别名AF488NHS活化酯
Canonical SMILES[NH2+]=C1C=CC2=C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)([O-])=O)=C5OC2=C1S(=O)([O-])=O
分子式C25H15N3O13S2Li2
分子量643.41
溶解度Soluble in DMSO
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Rossi et al (2012) Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 125 4405 PMID:22718347 |Burnette et al (2011) Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc.Natl.Acad.Sci.USA. 108 21081 PMID:22167805 |van de Linde et al (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat.Protoc. 6 991 PMID:21720313 |Iwata et al (2005) Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 102 13135 PMID:16141322 |Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID:10449539