Swinholide A 是结合肌动蛋白的一种海洋聚酮化合物，可将肌动蛋白二聚化，Kd约为 50 nM。 Swinholide A 是一种破坏海洋毒素的微丝，可稳定肌动蛋白二聚体并切断肌动蛋白丝。Swinholide A 破坏细胞的肌动蛋白细胞骨架。具有抗真菌 (antifungal)活性。
| 生物活性 | Swinholide A is theactin-binding marine polyketide and dimerizes actin with theKdof ~ 50 nM[1]. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. Swinholide A disrupts the actincytoskeletonof cells.Antifungalactivity[2]. |
体外研究
(In Vitro) | Swinholide A, first isolated from the Okinawan marine spongeTheonella swinhoei, dimerizes actin[1].
Swinholide A, isolated from the marien spongeTheonella swinhoei, is highly cytotoxic to a variety of cancer cell lines[2].
Swinholide A disrupts the actin cytoskeleton of cells grown in culture, sequesters actin dimers in vitro in both polymerizing and non-polymerizing buffers with a binding stoichiometry of one swinholide A molecule per actin dimer, and rapidly severs F-actin in vitro with high cooperativity[2].
Cell Viability Assay[2] | Cell Line: | Balb/c 3T3 and Swiss 3T3 cells | | Concentration: | 5-100 nM | | Incubation Time: | 1-24 h | | Result: | Exponentially growing cells exposed to 10 nM for 24 h became arborized with diffuse cytoplasmic staining and fluorescent punctate structures.
Partial cell retraction or arborization and diminution of microfilament bundles began after 2-4 h, with complete loss of stress fibers by 5-7 h at concentrations of 10-50 nM.
Caused rounding of cultured mouse embryo 3T3 fibroblast cells within 1 h at concentration of 80 nM. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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