Harringtonolide

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Harringtonolide

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

64761-48-4

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
50mg	电议

产品介绍

Harringtonolide 是一种有效的RACK1抑制剂，在 A375 细胞中IC₅₀=39.66 μM。Harringtonolide 通过影响FAK和RACK1的相互作用来抑制上皮-间充质转化 (EMT) 过程和细胞增殖。Harringtonolide 具有抑制植物生长、抗病毒、抗炎、抗增殖活性。

生物活性

Harringtonolide is a potentRACK1inhibitor (IC₅₀=39.66 μM in A375 cells). Harringtonolide inhibits the epithelial-mesenchymal transition (EMT) process and cell proliferation by affecting the interaction betweenFAKandRACK1. Harringtonolide has plant growth inhibitory, antiviral, anti-inflammatory, and antiproliferation activities^[1].

IC₅₀& Target

IC₅₀: 39.66 μM (RACK1) in A375 cells^[1]

体外研究
(In Vitro)

Harringtonolide (0-50 μM; 24 hours) exhibits good antiproliferation activity in A375 cells with IC₅₀of 39.66 μM^[1].
Harringtonolide (0-20 μM; 1 hour) restrains the proteolysis of RACK1 by Pronase, and protects temperature-dependent degradation of RACK1^[1].
Harringtonolide (0-4 μM; 24 hours) suppresses the phosphorylation of FAK dose-dependently and inhibits Src and STAT3, the downstream proteins of FAK^[1].
Harringtonolide (0-4 μM; 24 hours) dose-dependently inhibits the RACK1–FAK interaction in A375 cells^[1].

Cell Proliferation Assay

Cell Line:	A375 melanoma cells^[1]
Concentration:	0-50 μM
Incubation Time:	24 hours
Result:	Showed good antiproliferation activity with IC₅₀of 39.66 μM.

Western Blot Analysis

Cell Line:	A375 melanoma cells^[1]
Concentration:	0, 0.5, 1, 2, 4 μM
Incubation Time:	24 hours
Result:	Suppressed the phosphorylation of FAK dose-dependently and inhibited Src and STAT3, the downstream proteins of FAK.

分子量

310.34

性状

Solid

Formula

C₁₉H₁₈O₄

CAS 号

64761-48-4

结构分类

Ketones, Aldehydes, Acids

来源

Plants
Cephalotaxaceae
harringtonia

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)