体外研究
(In Vitro) | Moroidin (compound 1) (0-80 μM; 24, 48, or 72 h) has cytotoxicity to cancer cells with IC50values of 3.2, 8.3, 9.6, 5.2 and 9.9 μM in A549, H1299, U87, U251 and HCT116 cells, respectively[1].
Moroidin (0.01-100 μM; 24 h, 48 h, 72 h) interferes with microtubule polymerization and decreases β-tubulin protein levels in A549 cells[1].
Moroidin (0-20 μM; 48 h) arrests the cell cycle in the G2/M phase and induces apoptosis in A549 cells[1].
Moroidin (0.5, 1, 2 μM; 0, 24, 48 h) represses the migration and invasion of A549 cells[1].
Cell Viability Assay[1] | Cell Line: | A549 cells | | Concentration: | 0.01-100 μM | | Incubation Time: | 24 h, 48 h, 72 h | | Result: | Is not as potent as colchicine in inhibiting tubulin polymerization and proliferation in A549 cells. |
Western Blot Analysis[1] | Cell Line: | A549 cells | | Concentration: | 2.5 μM, 5 μM, 10μM | | Incubation Time: | | | Result: | Resulted in a dosedependent decrease in the protein level of tubulin in A549 cells. |
Apoptosis Analysis[1] | Cell Line: | A549 cells | | Concentration: | 0-20 μM | | Incubation Time: | 48 h | | Result: | Increased significantly the percentage of apoptosis. |
Cell Cytotoxicity Assay[1] | Cell Line: | A549, H1299, U87, U251, HCT116 and MCF-7 cells | | Concentration: | 0-80 μM | | Incubation Time: | 24, 48, or 72 h | | Result: | Inhibited the proliferation of cancer cells in vitro and exhibited the high sensitivity with the low IC50value in A549 cells. |
Cell Cycle Analysis[1] | Cell Line: | A549 cells | | Concentration: | 0-20 μM | | Incubation Time: | 48 h | | Result: | Caused a dose-dependent increase in the proportion of cells in the G2/M phase, a corresponding decrease in the ratio of cells in the G1 phase. |
Immunofluorescence[1] | Cell Line: | A549 cells | | Concentration: | 2.5 μM, 5 μM | | Incubation Time: | 48 h | | Result: | Resulted in a dose-dependent reduction and fragmentation of the microtubule network, accompanied by cell morphology alteration and fluorescence signal attenuation. |
Cell Invasion Assay[1] | Cell Line: | A549 cells | | Concentration: | 0.5, 1, 2 μM | | Incubation Time: | 0, 24, 48 h | | Result: | Decreased the invasion number of A549 cells with dose-dependentance. |
Cell Migration Assay[1] | Cell Line: | A549 cells | | Concentration: | 0.5, 1, 2 μM | | Incubation Time: | 0, 24, 48 h | | Result: | Showed a significant inhibition in migration of A549 cells, the migrated number of A549 cells was reduced significantly in a dosedependent manner. |
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