Grape seed extract 是一种天然产物，具有抗炎和抗增殖作用。Grape seed extract 对脂肪代谢酶、胰脂肪酶和脂蛋白脂肪酶有抑制作用。Grape seed extract 能诱导人类结肠直肠癌细胞凋亡 (apoptotic)。
| 生物活性 | Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract inducesapoptoticin human colorectalcancercells[1][2][3]. |
体外研究
(In Vitro) | Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells[3].
Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells[3].
Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells[3].
Cell Proliferation Assay[3] | Cell Line: | HT29 cells, LoVo cells | | Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL | | Incubation Time: | 12 hours, 24 hours, 48 hours | | Result: | Inhibited cell growth in a dose- and a time-dependent manner. |
Cell Cycle Analysis[3] | Cell Line: | HT29 cells, LoVo cells | | Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL | | Incubation Time: | 12 hours, 24 hours, 48 hours | | Result: | Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases). |
Western Blot Analysis[3] | Cell Line: | HT29 cells, LoVo cells | | Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL | | Incubation Time: | 12 hours, 24 hours | | Result: | Modulates the protein levels of CDKIs, CDKs, and cyclins. |
Apoptosis Analysis[3] | Cell Line: | HT29 cells, LoVo cells | | Concentration: | 25 μg/mL, 50 μg/mL, 100 μg/mL | | Incubation Time: | 24 hours | | Result: | Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells. |
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体内研究
(In Vivo) | Grape seed extract (200 mg/kg; i.g.; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice[3].
Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors[3].
Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors[3].
| Animal Model: | Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft[3] | | Dosage: | 200 mg/kg | | Administration: | Oral gavage, 5 days/wk, for 8 weeks | | Result: | Showed time-dependent inhibition of tumor growth. |
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| Clinical Trial | |
| 性状 | |
| CAS 号 | |
| 中文名称 | 葡萄籽提取物； 葡萄籽精华； 葡萄籽萃取； 葡萄籽萃取物 |
| 结构分类 | |
| 来源 | - Plants
- Vitaceae
- Vitis viniferacv. Zalema
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| 溶解性数据 | In Vitro: DMSO : 6 mg/mL(Need ultrasonic) In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution
此方案可获得 ≥ 0.6 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 6.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 0.6 mg/mL (Infinity mM); Clear solution
此方案可获得 ≥ 0.6 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 6.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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