4′-Hydroxywogonin (8-Methoxyapigenin) 是一种黄酮类化合物，可以从Scutellaria barbata和Verbena littoralis等多种植物中分离得到。4′-Hydroxywogonin 通过 TAK1/IKK/NF-κB、MAPKs 和 PI3/AKT 信号通路具有抗炎活性。4′-Hydroxywogonin 通过干扰 PI3K/AKT 信号抑制血管生成。4′-Hydroxywogonin 抑制细胞增殖和诱导细胞凋亡 (apoptosis)。
| 生物活性 | 4′-Hydroxywogonin (8-Methoxyapigenin), a flavonoid, could be isolated from a variety ofplantsincludingScutellaria barbataandVerbena littoralis. 4′-Hydroxywogonin has anti-inflammatory activity via TAK1/IKK/NF-κB, MAPKs and PI3/AKT signaling pathways. 4′-Hydroxywogonin inhibits angiogenesis by disrupting PI3K/AKT signaling. 4′-Hydroxywogonin inhibits cell proliferation and inducesapoptosis[1][2][3]. |
体外研究
(In Vitro) | 4′-Hydroxywogonin (8-Methoxyapigenin; 0.5-15 μM; 0-24 h) has low cytotoxicity and inhibits NO and PGE2production in LPS-stimulated RAW 264.7 macrophages by suppression of iNOS and COX-2 expression[1].
4′-Hydroxywogonin (0.5-15 μM; 1 and 12 h) suppresses LPS-induced expression of pro-inflammatory cytokines in RAW 264.7 macrophages and suppresses LPS-induced activation of NF-κB[1].
4′-Hydroxywogonin (0.5-15 μM; 1 h) suppresses LPS-induced degradation of IκB-α and activation of IKK and TAK and suppresses the phosphorylation of MAPK and AKTin in RAW 264.7 macrophages[1].
4′-Hydroxywogonin (0.5-15 μM; 24 h) inhibits ROS production in LPS-stimulated RAW 264.7 macrophages[1].
4′-Hydroxywogonin (0-10 μg/mL; 24 h) reduces the viability of SW620 cells in a concentration- and time-dependent manner and decreases the mRNA and protein expression of vascular endothelial growth factor-A (VEGF-A), the predominant pro-angiogenic cytokine in tumor angiogenesis[2].
4′-Hydroxywogonin (24 h; SUP-B15 and Jurkat cells) induces apoptosis and decreases the expression of C-MYC, BCL-2 and cleaved caspase 3[3].
Cell Viability Assay[1] | Cell Line: | RAW 264.7 macrophages | | Concentration: | 0.5, 5 and 15 μM | | Incubation Time: | 24 hours | | Result: | Had low cytotoxicity in RAW 264.7 macrophages. |
Western Blot Analysis[1] | Cell Line: | RAW 264.7 macrophages | | Concentration: | 0.5, 5 and 15 μM | | Incubation Time: | 1 hours | | Result: | Attenuated the increase of iNOS and COX-2 mRNA expression induced by LPS in RAW 264.7 cells. |
Western Blot Analysis[1] | Cell Line: | RAW 264.7 macrophages | | Concentration: | 0.5, 5 and 15 μM | | Incubation Time: | 1 and 12 hours | | Result: | Reduced TNF-α, IL-6 and IL-1β mRNA expression in a dose-dependent manner.
Inhibited LPS-induced p65 phosphorylation and nuclear translocation.
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Western Blot Analysis[1] | Cell Line: | RAW 264.7 macrophages | | Concentration: | 0.5, 5 and 15 μM | | Incubation Time: | 1 hours | | Result: | Attenuated LPS induced IκB-α degradation.
Attenuated the phosphorylation of ERK1/2 and p38 in a dose-dependent manner.
Reduced the intensity of the TAK1/TAB1 band.
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Western Blot Analysis[2] | Cell Line: | SW620 cells | | Concentration: | 0.1, 1, and 10 μg/mL | | Incubation Time: | 24 hours | | Result: | Downregulated VEGF-Aexpression in colorectal cancer cells and suppressed angiogenesis. |
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体内研究
(In Vivo) | 4′-Hydroxywogonin (10 and 20 mg/kg; i.p.; male C57BL/6 mice) alleviates LPS-induced acute lung injury (ALI) in a mouse model[1].
| Animal Model: | Male C57BL/6 mice (6-8 weeks old; 20 g) with acute lung injury model[1] | | Dosage: | 10 and 20 mg/kg | | Administration: | Intraperitoneal injection, 12 and 1 h before LPS treatment | | Result: | Had potential protective effects against inflammation in LPS induced ALI mice.
Attenuated the degree of leukocyte infiltration.
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| 来源 | - Plants
- Verbenaceae
- Verbena littoralisH.B.K.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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