ZSTK474

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ZSTK474

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	475110-96-4
包装:	10mg, 50mg, 100mg

包装与价格：

产品介绍

ZSTK474能够抑制I型PI3K亚型，IC50为37 nM，对PI3Kδ作用最显著。

细胞实验
细胞系	panel of 39 human cancer cell lines
方法	Analysis of Cell Proliferation Inhibition The inhibition of cell proliferation was assessed by measuring changes in total cellular protein in a culture of each cell line in the JFCR panel of cell lines after 48 hours of drug treatment by use of a sulforhodamine B assay. The 50% growth inhibition (GI 50 ) value of the drug was calculated as described previously. The graphic representation of a drug’s mean differential growth inhibition for the cell line panel was based on a calcula-tion that used a set of GI 50 values, as described previously. To analyze the correlation between the mean graphs of drug A and drug B, the COMPARE computer algorithm was developed as previously described by Paull et al. The Pearson correla-tion coeffi cient between the mean graphs of drug A and drug B was calculated (n = 39).
浓度	0~10μM
处理时间	48 h

动物实验
动物模型	Human A549 lung cancer cells, PC-3 prostate cancer cells, and WiDr colon cancer cells tumor xenografts
配制	5% hydroxypropylcellulose in water
剂量	100, 200, or 400 mg/kg daily from days 0 to 13
给药处理	orally

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.3957 mL	11.9786 mL	23.9573 mL
5 mM	0.4791 mL	2.3957 mL	4.7915 mL
10 mM	0.2396 mL	1.1979 mL	2.3957 mL