| 包装 | 价格(元) |
| 1mg | 电议 |
| 10mg | 电议 |
| 50mg | 电议 |
DHPG ((RS)-3,5-DHPG) is an amino acid that is a selective potent agonist for group I mGluR (mGluR 1 and mGluR 5) and has no effect on Group II and III mGluR.
Cell lines | MUller cell |
Preparation Method | (RS)-3,5-DHPG treatment induced the purification of cultured rat retinal Muller cells. |
Reaction Conditions | (RS)-3,5-DHPG (10 μM) for 0.5-24 h. |
Applications | (RS)-3,5-DHPG treatment induced down-regulation of Kir4.1 protein in cell membrane compartments of Muller cells. |
Animal models | Adult male Sprague Dawley rats (250-280 g) |
Preparation Method | To determine mechanisms underlying the effects of (RS)-3,5-DHPG infusion, some animals received two consecutive infusions conducted 10 min apart. Rats in these groups received infusions of ACSF followed by ACSF, ACSF followed by (RS)-3,5-DHPG (50 μmol), ACSF followed by the group I mGluR antagonist MPEP, or MPEP followed by (RS)-3,5-DHPG (50 μmol), ACSF followed by the PKD1 inhibitor followed by (RS)-3,5-DHPG (50 μmol). |
Dosage form | 50 μmol (RS)-3,5-DHPG for 5 days |
Applications | Impairments of spatial memory induced by bilateral infusions of (RS)-3,5-DHPG (50 μmol) into the CA1 area. Dose-dependent changes in spatial memory induced by (RS)-3,5-DHPG infusion. Pre-infusion of MPEP or CID755673 prevented impairments of spatial memory induced by (RS)-3,5-DHPG infusion. |
| 产品描述 | DHPG ((RS)-3,5-DHPG) is an amino acid that is a selective potent agonist for group I mGluR (mGluR 1 and mGluR 5) and has no effect on Group II and III mGluR[1，6]. DHPG ((RS)-3,5-DHPG) is also a potent antagonist of mGluRs associated with phospholipase D[2]. (RS)-3,5-DHPG (10μM) treatment induced MUller cell gliosis, as evidenced by enhanced GFAP expression. In addition, (RS)-3,5-DHPG (10 and 100μM) treatment induced a transient decrease in Kir4.1 mRNA expression in the cells. Activation of mGluR I by (RS)-3,5-DHPG may decrease the number of functional Kir4.1 channels in purified cultured rat retinal MUller cells through modulating Kir4.1 protein and mRNA, thus contributing to MUller cell gliosis[5]. Impairments of spatial memory induced by bilateral infusions of (RS)-3,5-DHPG (50 μmol) into the CA1 area. Dose-dependent changes in spatial memory induced by (RS)-3,5-DHPG infusion. Pre-infusion of MPEP or CID755673 prevented impairments of spatial memory induced by (RS)-3,5-DHPG infusion[3]. Hippocampal GPR30 expression was reduced in middle-aged mice compared with young adult mice. a group I metabotropic glutamate receptor (mGluR) agonist (RS)-3,5-DHPG -induced long-term depression in mossy fiber-cornu ammonis 3 (MF-CA3) synapses but not SC-CA1 synapses was facilitated in brain slices from G1-treated middle-aged mice[4]. (RS)-3,5-DHPG elicited acute mechanical allodynia and thermal hyperalgesia in naIve mice from 1 h to 12 h following intrathecal (central/spinal) administration.The attenuation of intrathecal (RS)-3,5-DHPG -evoked acute pain by artesunate therapy in rats[7]. References: |
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