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Citric acid trisodium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Citric acid trisodium图片
CAS NO:68-04-2
包装与价格:
包装价格(元)
100mg电议
500mg电议
1 g电议
5 g电议
10 g电议

产品名称
无水柠檬酸钠
产品介绍
Citric acid trisodium 是一种天然防腐剂和食品酸味增强剂。Citric acid trisodium 诱导细胞凋亡 (apoptosis) 和细胞周期停滞在 G2/M 期和 S 期。Citric acid trisodium 通过降低抗氧化酶活性引起肝脏的氧化损伤。Citric acid trisodium 对小鼠造成肾毒性。
生物活性

Citric acid trisodium is a natural preservative and food tartness enhancer. Citric acid trisodium inducesapoptosisand cell cycle arrest at G2/M phase and S phase. Citric acid trisodium cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid trisodium causes renal toxicity in mice[1][2][3].

IC50& Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Citric acid trisodium (0-12.5 mM; 24 h) shows antiproliferative activity in a dose dependent manner[3].
Citric acid trisodium (12.5 mM; 72 h) induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner[3].
Citric acid trisodium (12.5 mM; 48 h) increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl[3].

Cell Viability Assay[3]

Cell Line:HaCaT cells
Concentration:0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time:24 h
Result:Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis[3]

Cell Line:HaCaT cells
Concentration:12.5 mM
Incubation Time:0, 12, 24, 48, 72 h
Result:Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner.

Western Blot Analysis[3]

Cell Line:HaCaT cells
Concentration:12.5 mM
Incubation Time:12, 24, 48 h
Result:Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.
体内研究
(In Vivo)

Citric acid trisodium (120, 240, and 480 mg/kg; i.p.) significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver[1].
Citric acid trisodium (120, 240, and 480 mg/kg; i.p.) induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes[1].
Citric acid trisodium (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) causes renal toxicity in mice[2].

Animal Model:20 g male Kunming mice[2]
Dosage:120, 240, 480 mg/kg
Administration:I.p.; weekly for 3 weeks
Result:T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.
Clinical Trial
分子量

258.07

性状

Solid

Formula

C6H5Na3O7

CAS 号

68-04-2

中文名称

无水柠檬酸钠

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 100 mg/mL(387.49 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.8749 mL19.3746 mL38.7492 mL
5 mM0.7750 mL3.8749 mL7.7498 mL
10 mM0.3875 mL1.9375 mL3.8749 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。