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BMS453
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
BMS453图片
CAS NO:166977-43-1
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
BMS-189453
产品介绍
BMS453 (BMS-189453) 是一种合成类维生素 A,是一种RARβ激动剂和RARα/RARγ拮抗剂。BMS453 主要通过诱导活性 TGFβ 来抑制乳腺细胞生长。
生物活性

BMS453 (BMS-189453), a synthetic retinoid, is aRARβagonist and aRARα/RARγantagonist. BMS453 inhibits breast cell growth predominantly through the induction of active TGFβ[1][2].

体外研究
(In Vitro)

BMS453 (1 μM; 11 hours; 184 and HMEC cells) treatment inhibits the proliferation of normal breast cell growth without significantly inducing apoptosis[2].
BMS453 (1 μM; 5 days; 184 and HMEC cells) treatment inhibits normal breast cell proliferation by causing G1 arrest[2].
BMS453 (1 μM; 24-72 hours; 184 cells) treatment induces Rb hypophosphorylation and decrease CDK2 kinase activity. BMS453 increases total p21 protein levels and CDK2-bound p21 protein, but does not change CDK4-bound p21[2].
BMS453 inhibits breast cell growth predominantly through the induction of active TGFβ[2].

Cell Proliferation Assay[2]

Cell Line:Normal human mammary epithelial cells (184 and HMEC)
Concentration:1 μM
Incubation Time:11 hours
Result:Inhibited3H-thymidine uptake in normal breast cells (184 and HMEC) by 40 %.

Cell Cycle Analysis[2]

Cell Line:Normal human mammary epithelial cells (184 and HMEC)
Concentration:1 μM
Incubation Time:5 days
Result:Increased the proportion of cells in G0/G1 phase and decreased the proportion of cells in S phase.

Western Blot Analysis[2]

Cell Line:184 cells
Concentration:1 μM
Incubation Time:24 hours, 48 hours, 72 hours
Result:Induced Rb hypophosphorylation and decrease CDK2 kinase activity.
分子量

380.48

性状

Solid

Formula

C27H24O2

CAS 号

166977-43-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 16.67 mg/mL(43.81 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.6283 mL13.1413 mL26.2826 mL
5 mM0.5257 mL2.6283 mL5.2565 mL
10 mM0.2628 mL1.3141 mL2.6283 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。