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Idebenone
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Idebenone图片
CAS NO:58186-27-9
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
艾地苯醌
产品介绍
Idebenone,一种线粒体保护剂,具有神经保护功效,可用于研究阿尔茨海默病、亨廷顿舞蹈病,可透过血脑屏障。Idebenone (氧化形式) 对星形胶质细胞匀浆中花生四烯酸的酶代谢具有抑制作用 (IC50=16.65 μM)。 Idebenone 是一种辅酶 Q10 类似物和抗氧化剂,在人多巴胺能神经母细胞瘤 SHSY-5Y 细胞中诱导细胞凋亡 (apoptotic)。
生物活性

Idebenone, a well-appreciated mitochondrial protectant, exhibits protective efficacy against neurotoxicity and can be used for the research of Alzheimer's disease, Huntington's disease. Idebenone (oxidised form) has a dose-dependent inhibitory effect on the enzymatic metabolism of arachidonic acid in astroglial homogenates (IC50=16.65 μM)[1]. Idebenone, a coenzyme Q10 analog and an antioxidant, inducesapoptoticcell death in the human dopaminergic neuroblastoma SHSY-5Y cells[2]. Idebenone quickly crosses the blood-brain barrier.

体外研究
(In Vitro)

Idebenone, a compound with protective efficacy against neurotoxicity both in in vitro and in in vivo models, exists in two different oxidative states: the ubiquinol-derivative (reduced idebenone) and the ubiquinone-derivative (oxidised idebenone)[1].
Idebenone (oxidised form) preferentially inhibits cyclooxygenase vs. lipoxygenase metabolism (IC50ratio lipoxygenase/cyclooxygenase: 3.22)[1].
Idebenone (oxidised form) behaves similarly as indomethacin and piroxicam—two typical anti-inflammatory agents[1].
Idebenone (oxidised form) inhibits total arachidonic acid metabolism, cyclooxygenase metabolism, lipoxygenase metabolism, lipoxygenase/cyclooxygenase with IC50s of 16.65±3.48, 14.44±2.99, 46.51±7.20, and 3.22 μM[1].
Idebenone (1-10 μM; for 24-72 h) has no effect on the cell viability of SHSY-5Y cells[2].
Idebenone (25 μM or higher concentrations; for 24-72 h) shows significant reduction in cell viability of SHSY-5Y cells[2]. Idebenone (30 μM) induces up-regulation of BAX expression and the caspase-3 activity[2].

Cell Viability Assay[2]

Cell Line:The human dopaminergic neuroblastma cell line, SHSY-5Y cells
Concentration:1, 3, 10, 15, 25, 30, and 90 μM
Incubation Time:24, 48, and 72 hours
Result:Had no apparent detrimental effects on cell viability as indicated by the absence of trypan blue-positive staining in the cells at concentrations of 1, 3, 10 μM.
Exhibited some degree of trypan blue-positive staining at 15 μM. Showed extensive trypan blue-positive staining at 25 μM and 30 μM.

RT-PCR[2]

Cell Line:SHSY-5Y cells
Concentration:10 μM, 30 μM
Incubation Time:72 hours
Result:The total RNA of BAX from SHSY-5Y cells exposed to 10 μM was not different from that of the untreated control cells.
The BAX expression in SHSY-5Y cells exposed to 30 μM was significantly up-regulated when compared with the untreated control cells and cells exposed to 10 μM.
体内研究
(In Vivo)

Idebenone (oxidised form) is a compound with protective efficacy against cerebrovascular disorders in vivo, including cerebral ischemia and hypertension-induced vascular lesions[1].

Clinical Trial
分子量

338.44

性状

Solid

Formula

C19H30O5

CAS 号

58186-27-9

中文名称

艾地苯醌

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL(295.47 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.9547 mL14.7737 mL29.5473 mL
5 mM0.5909 mL2.9547 mL5.9095 mL
10 mM0.2955 mL1.4774 mL2.9547 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.39 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.39 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (7.39 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。