Sparstolonin B 作为选择性TLR2和TLR4拮抗剂，选择性阻断 TLR2 和 TLR4 介导的炎症信号传导。Sparstolonin B 具有抗HIV和抗癌活性。
| 生物活性 | Sparstolonin B acts as a selectiveTLR2andTLR4antagonist and selectively blocks TLR2- and TLR4-mediated inflammatory signaling. Sparstolonin B has anti-HIVand anticancer activities[1][2]. |
| IC50& Target[1][2] | |
体外研究
(In Vitro) | Sparstolonin B (1-20 μM; 2-4 days) inhibits cell growth and viability of neuroblastoma cells[3].
Sparstolonin B inhibits TLR ligand-induced cytokine expression in mouse macrophages. Sparstolonin B inhibits MyD88 recruitment to TLR4 and TLR2[1].
Sparstolonin B generates reactive oxygen species (ROS) in neuroblastoma cells. Sparstolonin B reduces expression of N-myc in neuroblastoma cells[3].
Cell Viability Assay[3] | Cell Line: | SH-SY5Y, IMR-32, NGP, SKNF-1 and SK-N-BE(2) cells | | Concentration: | 1 μM, 5 μM, 10 μM or 20 μM | | Incubation Time: | 2-4 days | | Result: | Effectively and dose-dependently inhibits the viability of all neuroblastoma cell lines after 2 days (SH-SY5Y and IMR-32), 3 days (NGP cells) or 4 days (SKNF-1 and SK-N-BE(2) cells) treatment. |
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体内研究
(In Vivo) | Sparstolonin B (100 μg/mouse; i.p.) suppresses LPS-provoked inflammation in mice[1].
| Animal Model: | 5-6-week-old male C57Bl/6 mice (body weight 18-20 g)[1] | | Dosage: | 100 μg/mouse | | Administration: | I.p. | | Result: | Significantly lower TNFα and IL-1β expression levels in LPS-induced sepsis mouse model. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 10 mg/mL(37.28 mM;Need ultrasonic and warming) 配制储备液 | 1 mM | 3.7283 mL | 18.6414 mL | 37.2828 mL | | 5 mM | 0.7457 mL | 3.7283 mL | 7.4566 mL | | 10 mM | 0.3728 mL | 1.8641 mL | 3.7283 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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