FSL-1 TFA 是细菌衍生的一种 Toll 样受体 2/6 (TLR2/6) 激动剂，可增强对 HSV-2 感染的抵抗力。FSL-1 TFA 通过TLR2和NF-κB/AP-1信号通路诱导MMP-9产生。
| 生物活性 | FSL-1 TFA, a bacterial-derivedtoll-like receptor2/6 (TLR2/6) agonist, enhances resistance to experimental HSV-2infection[1]. FSL-1 TFA inducesMMP-9production throughTLR2andNF-κB/AP-1signaling pathways in monocytic THP-1 cells[2]. |
| IC50& Target[1][2] | |
体外研究
(In Vitro) | FSL-1 significantly reduces HSV-2 replication in human vaginal epithelial cells (EC)[1].
FSL-1 induces significant resistance to experimental genital HSV-2 infection through elaboration of a specific cytokine response profile[1].
FSL-1 (50 ng/mL, 24 hours) induces MMP-9 expression at both mRNA and protein levels in human monocytic THP-1 cells[2].
FSL-1 activates the MAP kinase/NF-κB signaling pathway[2].
Cell Viability Assay[1] | Cell Line: | V11I, V12I or V19I immortalized human vaginal EC | | Concentration: | 6 μg or 0.1 μg | | Incubation Time: | Added at 24, 6 or just prior to HSV-2 inoculation (104pfu/well) | | Result: | The 6 μg does produced significant reductions when delivered at 24 or 6 h prior to HSV-2 inoculation. The 0.1 μg dose produced reduced HSV-2 replication at 24 or 6 h prior to viral challenge. |
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体内研究
(In Vivo) | FSL-1 application significantly protectes against genital HSV-2 challenge in mice[1].
| Animal Model: | Female Swiss-Webster mice (weighing 20-25 g)[1] | | Dosage: | 2 or 6 μg | | Administration: | Delivered vaginally using a positive displacement pipet, prior to or following viral challenge as specified for each experiment. | | Result: | The 2 μg does delivered 6 h prior to HSV-2 challenge increased the ID50 (260 pfu) and LD50 (660 pfu) by 10-fold compared to DPBS vehicle control.
The single 6 μg dose produced significantly improved outcomes compared to DPBS vehicle application. |
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| 分子量 | |
| 性状 | |
| Formula | |
| Sequence Shortening | S-(2, 3-Bispalmitoyloxypropyl)-CGDPKHPKSF |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | |
| 溶解性数据 | In Vitro: H2O : 50 mg/mL(28.09 mM;Need ultrasonic) 配制储备液 | 1 mM | 0.5617 mL | 2.8087 mL | 5.6174 mL | | 5 mM | 0.1123 mL | 0.5617 mL | 1.1235 mL | | 10 mM | 0.0562 mL | 0.2809 mL | 0.5617 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months。-80℃ 储存时，请在 6 个月内使用。 |
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