Cyclic-di-GMP sodium 是一种STING激动剂，也是细菌第二信使，协调细菌生长和行为的不同方面，包括运动能力、毒力、生物膜的形成和细胞周期的进展。Cyclic-di-GMP sodium 具有抗癌细胞增殖活性，还可诱导CD4受体表达升高和细胞周期停滞。Cyclic-di-GMP sodium 可用于癌症的研究。
| 生物活性 | Cyclic-di-GMP sodium is aSTINGagonist and abacterialsecond messengerthat coordinates different aspects ofbacterialgrowth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP sodium has anti-cancer cell proliferation activity and also induces elevatedCD4receptorexpression and cell cycle arrest. Cyclic-di-GMP sodium can be used incancerresearch[1][2][3][4]. |
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体外研究
(In Vitro) | Cyclic-di-GMP sodium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
Cyclic-di-GMP sodium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
Cyclic-di-GMP sodium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].
Cell Proliferation Assay[1] | Cell Line: | H508 cells | | Concentration: | 0.5-50 μM | | Incubation Time: | 5 days | | Result: | Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation. |
Cell Viability Assay[2] | Cell Line: | Jurkat cells | | Concentration: | 50 μM | | Incubation Time: | 24 h | | Result: | Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner. |
Cell Cycle Analysis[2] | Cell Line: | Jurkat cells | | Concentration: | 50 μM | | Incubation Time: | 24 h | | Result: | Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%. |
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体内研究
(In Vivo) | Cyclic-di-GMP sodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].
| Animal Model: | C57BL/6 (B6) mice (8- to 10-week-old)[3]. | | Dosage: | 100 μg/per | | Administration: | Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax. | | Result: | Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 μg hgp100 or 100 μg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax. |
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| Formula | |
| 结构分类 | - Ketones, Aldehydes, Acids
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| 来源 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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