Cyclic-di-GMP 是一种STING激动剂，也是细菌第二信使，协调细菌生长和行为的不同方面，包括运动能力、毒力、生物膜的形成和细胞周期的进展。Cyclic-di-GMP 具有抗癌细胞增殖活性，还可诱导CD4受体表达升高和细胞周期停滞。Cyclic-di-GMP 可用于癌症的研究。
| 生物活性 | Cyclic-di-GMP is aSTINGagonist and abacterialsecond messengerthat coordinates different aspects ofbacterialgrowth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP has anti-cancer cell proliferation activity and also induces elevatedCD4receptorexpression and cell cycle arrest. Cyclic-di-GMP can be used incancerresearch[1][2][3][4]. |
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体外研究
(In Vitro) | Cyclic-di-GMP (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells[1].
Cyclic-di-GMP (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells[2].
Cyclic-di-GMP (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells[2].
Cell Proliferation Assay[1] | Cell Line: | H508 cells | | Concentration: | 0.5-50 μM | | Incubation Time: | 5 days | | Result: | Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation. |
Cell Viability Assay[2] | Cell Line: | Jurkat cells | | Concentration: | 50 μM | | Incubation Time: | 24 h | | Result: | Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner. |
Cell Cycle Analysis[2] | Cell Line: | Jurkat cells | | Concentration: | 50 μM | | Incubation Time: | 24 h | | Result: | Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%. |
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体内研究
(In Vivo) | Cyclic-di-GMP (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax[3].
| Animal Model: | C57BL/6 (B6) mice (8- to 10-week-old)[3]. | | Dosage: | 100 μg/per | | Administration: | Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax. | | Result: | Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 μg hgp100 or 100 μg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC).
Enhanced the anti-tumor activity of TriVax. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: H2O : ≥ 25 mg/mL(36.21 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 1.4484 mL | 7.2421 mL | 14.4841 mL | | 5 mM | 0.2897 mL | 1.4484 mL | 2.8968 mL | | 10 mM | 0.1448 mL | 0.7242 mL | 1.4484 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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