Parstatin(human) TFA 是具有细胞通透性的PAR-1凝血酶受体的肽类激动剂，是一种有效的血管生成抑制剂。
| 生物活性 | Parstatin(human) TFA, a cell-penetratingPAR-1thrombinreceptoragonist peptide, is a potent inhibitor of angiogenesis[1][2]. |
| IC50& Target | |
体外研究
(In Vitro) | Parstatin (0-10 μM) increases recovery of LVDP in a concentration-dependent manner. The optimal concentration was 1 μM which produced a 23% recovery of LVDP[2].
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体内研究
(In Vivo) | Parstatin (single dose, 1-25 μg/kg, iv) administered prior to ischaemia confers immediate cardioprotection by recruiting the Gi-protein activation pathway including p38 MAPK, ERK1/2, NOS, and KATP channels. Parstatin exerts effects on both the cardiomyocytes and the coronary circulation to induce cardioprotection. This suggests a potential therapeutic role of parstatin in the treatment of cardiac injury resulting from ischaemia and reperfusion[1].
| Animal Model: | Male Sprague–Dawley rats at 8 weeks of age (250-300 g)[1]. | | Dosage: | 1-25 μg/kg. | | Administration: | IV injected 15 min prior to ischaemia. | | Result: | A significant decrease in infarct size was detected with the 5-15 μg/kg doses with 10 μg/kg as the optimal dose. These hearts had an infarct size of 46 ± 3% of the area at risk, which is a 26% reduction in infarct size compared with the control. |
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| 分子量 | |
| Formula | |
| Sequence Shortening | MGPRRLLLVAACFSLCGPLLSARTRARRPESKATNATLDPR |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Solvent & Solubility | In Vitro: H2O Peptide Solubility and Storage Guidelines: 1. Calculate the length of the peptide. 2. Calculate the overall charge of the entire peptide according to the following table: | | Contents | Assign value | | Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 | | Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 | | Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution: | Overall charge of peptide | Details | | Negative (<0) | 1. Try to dissolve the peptide in water first.
2. If water fails, add NH4OH (<50 μL).
3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. | | Positive (>0) | 1. Try to dissolve the peptide in water first.
2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. | | Zero (=0) | 1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
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