Orexin B,human TFA

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Orexin B,human TFA

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
500 μg	电议
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品名称

Human orexin B TFA

产品介绍

Orexin B, human (TFA) 是Orexin受体的内源性激动剂，与OX1和OX2结合的K_i值分别为 420 和 36 nM。

生物活性

Orexin B, human (TFA) is an endogenous agonist atOrexinreceptor withK_is of 420 and 36 nM for OX1 and OX2, respectively.

IC₅₀& Target

Ki: 420 nM (OX1), 36 nM (OX2)^[1].

体外研究
(In Vitro)

Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor^[1].
Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC₅₀of 36 nM in the binding assay and an EC₅₀of 60 nM in the [Ca²⁺]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC₅₀in the competitive binding assay and the EC₅₀in the [Ca²⁺]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively^[2].

体内研究
(In Vivo)

Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively^[2].

Animal Model:	Male Wistar rats (180-200 g)^[2].
Dosage:	3 nM and 30 nM.
Administration:	Administered in a 5 mL bolus through a catheter placed in the left lateral ventricle in early light phase.
Result:	Significantly augmented food intake.

分子量

3013.36

性状

Solid

Formula

C₁₂₃H₂₁₂N₄₄O₃₅S.C₂HF₃O₂

Sequence

Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2

Sequence Shortening

RSGPPGLQGRLQRLLQASGNHAAGILTM-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.