体外研究
(In Vitro) | Treatment of Mel-Ab cells with Terrein (10-100 μM) for 4 days significantly reduces melanin levels in a dose-dependent manner. Terrein reduces melanin synthesis by reducing tyrosinase production via ERK activation[1].
Terrein (5-500 μM; 24 and 48 hours) reduces the viability of these cells in concentration- and time-dependent manners[2].
Terrein (150, 250, 500μM; 24 hours) induces programmed cell death in both invasive and non-invasive breast cancer cell lines. Thus, the optimal non-toxic concentrations of Terrein (25 and 75 μM) were used for subsequent experiments[2].
Terrein (25 and 75 μM) significantly decrease the mRNA levels of both MMP-2 and MMP-9 in MDA-MB-231 cells. Terrein (75 μM) significantly decrease the mRNA levels of both MMP-2 and MMP-9 in MCF-7 cells[2].
Terrein (75 μM) inhibits the expression of RhoA, RhoB, p-Rac1 in MCF-7 cells. Terrein (75 μM) inhibits the expression of RhoB, p-Rac1 in MDA-MB-231 cells[2].
Terrein (10, 30, and 100 μM) inhibits the production of virulence factors such as elastase, pyocyanin, and rhamnolipid, as well as biofilm formation in P. aeruginosa PAO1 and PA14 strains[3].
Cell Viability Assay[2] | Cell Line: | MCF-7 and MDA-MB-231 cells | | Concentration: | 5, 25, 50, 75, 100, 150, 250, 500 μM | | Incubation Time: | 24 and 48 hours | | Result: | The IC50s for MCF-7 and MDA-MB-231 cells after 24 h of incubation were 2.34 mM and 700 μM, respectively. After 48 h of incubation, the IC50s are 244.3 μM for MCF-7 and 244.5 μM for MDA-MB-231 cells. |
Apoptosis Analysis[2] | Cell Line: | MCF-7 and MDA-MB-231 cells | | Concentration: | 25, 75, 150, 250, 500 μM | | Incubation Time: | 24 hours | | Result: | The percentages of apoptotic cells after 25 and 75 μM treatment were 7.52 and 5.82%, respectively for MCF-7 cells (9.94% for the control), and 15.77 and 15.82%, respectively for MDA-MB-231 cells (8.79% for the control). |
RT-PCR[2] | Cell Line: | MCF-7 and MDA-MB-231 cells | | Concentration: | 25, 75 μM | | Incubation Time: | | | Result: | Decreased the mRNA levels of both MMP-2 and MMP-9 in a concentration-dependent manner. |
Western Blot Analysis[2] | Cell Line: | MCF-7 and MDA-MB-231 cells | | Concentration: | 75 μM | | Incubation Time: | 6, 12 and 24 hours | | Result: | RhoA and RhoB protein levels were significantly decreased after 6 h of 75 μM treatment in MCF-7 cells.
Phosphorylated (p)-Rac1 was also slightly decreased at 12 h. However, RhoC and Cdc42 levels remained unchanged.
RhoB protein levels and Rac1 phosphorylation were also decreased in MDA-MB-231 cells at 12 and 24 h, respectively. |
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