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W146
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
W146图片
CAS NO:909725-61-7
包装:500 μg

产品介绍
W146 是 1-磷酸-鞘氨醇受体亚型 1 (S1PR1) 的选择性拮抗剂,EC50值为 398 nM。
生物活性

W146 is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50value of 398 nM.

IC50& Target

EC50: 398 nM (S1PR1)[1].

体外研究
(In Vitro)

W146 is a S1PR1 antagonist with a Kiof ~70-80 nM[1].
W146 pretreatment significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146[2].

Apoptosis Analysis[2]

Cell Line:Endothelial progenitor cells (EPCs).
Concentration:10 μM.
Incubation Time:30 min before the addition of S1P.
Result:Increases activated cleaved caspase-3 levels.
体内研究
(In Vivo)

W146 (5 mg/kg, ip, prior to AMD3100 administration) pre-treatment shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone[3]
The W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization. Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice[3].

Animal Model:Mice(4-6-week-old)[3]
.
Dosage:5 mg/kg.
Administration:IP, 1 hour prior to AMD3100 (ADM) administration.
Result:Significantly increased in KSL-HSPC mobilization compared to that in mice pretreated with dextran followed by AMD3100 administration.
分子量

342.37

性状

Solid

Formula

C16H27N2O4P

CAS 号

909725-61-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month