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Kinetensin(human)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Kinetensin(human)图片
包装:1mg
市场价:840元

产品介绍
Kinetensin (human) 是一种从经胃蛋白酶处理的人血浆中分离出来的神经降压素样肽。

Cell experiment:

Rat peritoneal mast cells are incubated with kinetensin at 37℃ for 10 min. The incubation is stopped by the addition of 1.8 mL of ice-cold buffered saline and cells are separated from supernatant by centrifugation. Histamine release is expressed as per cent of total mast cell histamine[2].

Animal experiment:

Rats[2]Anesthetized Sprague-Dawley rats are given 25I-albumin i.v. Samples are then injected intradermally in 5x2 spots on the back and comprised saline as a control or kinetensin in different doses in 100 μL saline. After 20 rain, skin biopsies of 7 mm diameter are cut out, weighed and transferred to a gamma-counter. Results are expressed as: (counts per min (cpm) in tissue per gram wet weight/cpm in plasma per mL plasma)[2].

产品描述

Kinetensin is a neurotensin-like peptide isolated from pepsin-treated human plasma.

The peptide kinetensin isolated from pepsin-treated human plasma induces a dose-dependent release of histamine when exposed to rat peritoneal mast cells. The threshold concentration is around 1 μM, the ED50 is 10 μM, and the optimal concentration of between 100 to 1000 μM released 80% of the total histamine. Kinetensin is 10 to 100 times less potent than neurotensin and equipotent with the opioid peptide dynorphin. The histamine release is clearly temperature-dependent, with no release occurring at 0 or 45 ℃ and with an optimum around 37 ℃. The histamine release is significantly reduced in the absence of extracellular calcium[2].

Kinetensin also induces a dose-dependent increase in vascular permeability when injected intradermally into rats[2].

References:
[1]. Mogard MH, et al. The amino acid sequence of kinetensin, a novel peptide isolated from pepsin-treated human plasma: homology with human serum albumin, neurotensin and angiotensin. Biochem Biophys Res Commun. 1986 May 14;136(3):983-8.
[2]. Sydbom A, et al. Stimulation of histamine release by the peptide kinetensin. Agents Actions. 1989 Apr;27(1-2):68-71.