Ankaflavin 是从 Monascus-Fermented 红米中提取得到的，是一种具有口服活性的PPARγ的激动剂。Ankaflavin 具有选择性的细胞毒性作用，诱导癌细胞凋亡（apoptosis）。Ankaflavin 具有抗炎、抗癌、抗动脉粥样硬化和降血脂的作用。
| 生物活性 | Ankaflavin, isolated from Monascus-Fermented red rice, is an orally activePPARγagonist. Ankaflavin exhibits selective cytotoxic effect and induces cell death throughapoptosisoncancercells. Ankaflavin has anti-inflammatory, anti-cancer, antiatherosclerotic, and hypolipidemic effects[1][2]. |
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体外研究
(In Vitro) | Ankaflavin (0-50 μg/mL, 48 h) shows cytotoxicity against cancer cells with no significant toxicity toward normal cells[1].
Ankaflavin (0-30 μg/mL, 0-48 h) arrests Hep G2 cell cycle at sub-G1 phase in a dose- and time-dependent manner[1].
Ankaflavin (25 μg/mL, 48 h) induces Hep G2 cell apoptosis[1].
Cell Cytotoxicity Assay[1] | Cell Line: | A549, Hep G2, MRC-5 and WI-38 | | Concentration: | 1, 10, 25, and 50 μg/mL | | Incubation Time: | 48 h | | Result: | Showed cytotoxicity against A549 and Hep G2 cells in a dose-dependent manner with no significant toxicity toward normal cells (MRC-5 and WI-38). |
Cell Cycle Analysis[1] | Cell Line: | Hep G2 cells | | Concentration: | 15, 20, 25, and 30 μg/mL | | Incubation Time: | 12, 24, 36, and 48 h | | Result: | Induced a distinct sub-G1 peak in Hep G2 cells in a dose- and time-dependent manner. |
Apoptosis Analysis[1] | Cell Line: | Hep G2 cells | | Concentration: | 25 μg/mL | | Incubation Time: | 48 h | | Result: | Exhibited significant chromatin condensation (fluorescent spot) through Hoechst staining. |
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体内研究
(In Vivo) | Ankaflavin (10 mg/kg; p.o.; daily for 28 days) shows antidiabetic and anti-inflammatory activity, improves liver function and pancreatic function[2].
| Animal Model: | Wistar rats (4 weeks of age), diabetes was induced by treating them with Methylglyoxal (MG) (600 mg/kg; oral) for 4 weeks[2] | | Dosage: | 10 mg/kg | | Administration: | Oral administration for 28 days | | Result: | Exerted PPARγ agonist activity. Effectively reduced AGE (advanced glycation end-products) levels in serum, liver, and pancreas of MG-induced rats. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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