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Imipramine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Imipramine图片
CAS NO:50-49-7
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
米帕明
产品介绍
Imipramine 是一种具有口服活性的叔胺类三环抗抑郁药。Imipramine 是一种具有抗肿瘤活性的Fascin1抑制剂。Imipramine 也抑制 5-羟色胺转运体 (serotonin transporter),其IC50值为 32 nM。Imipramine 刺激 U-87MG 胶质瘤细胞自噬(autophagy),诱导 HL-60 细胞凋亡(apoptosis)。Imipramine 具有神经保护和免疫调节作用。
生物活性

Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is aFascin1inhibitor with antitumor activities. Imipramine also inhibitsserotonin transporterwith anIC50value of 32 nM. Imipramine stimulates U-87MG glioma cellsautophagyand induces HL-60 cellapoptosis. Imipramine shows neuroprotective and immunomodulatory effects[1][2][3][4][5].

IC50& Target

Fascin1, Serotonin Transporter, Autophagy, Apoptosis[1][2][3][5]
IC50: 32 nM (human placental serotonin transporter)[5]

体外研究
(In Vitro)

Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability[1].
Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h)[1].
Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells[2].
Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy[2].
Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis[3].

Cell Viability Assay[1]

Cell Line:DLD-1, HCT-116, and SW-480
Concentration:0.5-300 μM
Incubation Time:3 days
Result:Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.

Cell Migration Assay[1]

Cell Line:DLD-1, HCT-116, and SW-480
Concentration:20 μM
Incubation Time:7 h
Result:Produced a remarkable inhibition of migration in all assayed cell lines.

Cell Invasion Assay[1]

Cell Line:HCT-116
Concentration:20 μM
Incubation Time:48 h
Result:Inhibited cell invasion through Matrigel.

Western Blot Analysis[2]

Cell Line:U-87MG
Concentration:50 μM
Incubation Time:0, 15, 30, 60, 120 and 240 min
Result:Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.

Cell Autophagy Assay[2]

Cell Line:U-87MG
Concentration:60 μM
Incubation Time:24 h
Result:Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.

Apoptosis Analysis[3]

Cell Line:HL-60
Concentration:80 μM
Incubation Time:24 h
Result:Induced cell apoptosis.
体内研究
(In Vivo)

Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice[4].

Animal Model:Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)[4]
Dosage:20 mg/kg or 15 mg/kg
Administration:Intraperitoneal injection or oral administration, daily for 24 days
Result:Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.
Clinical Trial
分子量

280.41

Formula

C19H24N2

CAS 号

50-49-7

中文名称

米帕明

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.