Ro 08-2750

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Ro 08-2750

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

37854-59-4

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
1mg	电议
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议
200mg	电议

产品介绍

Ro 08-2750 是一种非肽、可逆的NGF抑制剂，能直接结合 NGF，IC₅₀值为 ~ 1 μM。Ro 08-2750 能抑制 NGF 结合 p75^NTR的优于 TRKA。Ro 08-2750 是选择性的MSI RNA 结合抑制剂，其作用的IC₅₀值为 2.7 μM。

生物活性

Ro 08-2750 is a non-peptide and reversiblenerve growth factor (NGF)inhibitor which binds to NGF, and with anIC₅₀of ~ 1 μM. Ro 08-2750 inhibits NGF binding to p75^NTRselectively overTRKA^[1]. Ro 08-2750 is a selective_{MSI RNA-binding}activity inhibitor, with anIC₅₀of 2.7 μM^[3].

IC₅₀& Target

IC50: ~1 μM (NGF)^[1], 2.7 μM (MSI RNA-binding)^[3]

体外研究
(In Vitro)

Ro 08-2750 binds to the NGF dimer thereby probably inducing a change in its conformation such that NGF cannot bind to p75^NTRanymore^[2].
Ro 08-2750 (10 nM) completely rescues cells from undergoing NGF-induced SK-N-MC 103 cells death^[2].
Ro 08-2750 (5-10 μM; 8 hours) increases differentiation and apoptosis in myeloid leukemia cells^[3].
Ro 08-2750 inhibits survival of human AML lines and patient cells^[3].
Ro 08-2750 inhibits MSI2 RNA-binding and alters MSI2 gene signature^[3].

Apoptosis Analysis^[3]

Cell Line:	MLL-AF9 + BM cells
Concentration:	5 μM, 10 μM
Incubation Time:	8 hours
Result:	Increased apoptosis.

体内研究
(In Vivo)

Ro 08-2750 (13.75 mg/kg; i.p.) inhibits leukemogenesis in a myeloid leukemia model in vivo^[3].

Animal Model:	C57BL/6 wild type mice (10-12-weeks-old), MLL-AF9 murine leukemia model^[3]
Dosage:	13.75 mg/kg
Administration:	Intraperitoneal injection, at days 1, 4, 7, 10, and 13 (one day on, two days off drug)
Result:	Inhibited c-MYC levels and reduced disease burden.

分子量

270.24

性状

Solid

Formula

C₁₃H₁₀N₄O₃

CAS 号

37854-59-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 4 mg/mL(14.80 mM;ultrasonic and warming and heat to 80℃)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.7004 mL	18.5021 mL	37.0041 mL
5 mM	0.7401 mL	3.7004 mL	7.4008 mL
10 mM	0.3700 mL	1.8502 mL	3.7004 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。