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Meisoindigo
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Meisoindigo图片
CAS NO:97207-47-1
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
2mg电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
甲异靛
Dian III
N-Methylisoindigotin
Natura-α
产品介绍
Meisoindigo (Dian III) 是 Indirubin (HY-N0117) 的衍生物,可以造成急性髓细胞性白血病 (AML) 细胞周期在 G0/G1 期停滞,并诱导凋亡。Meisoindigo 具有很高的抗肿瘤活性。
生物活性

Meisoindigo (Dian III), a derivative of Indirubin (HY-N0117), halts the cell cycle at the G0/G1 phase and inducesapoptosisin primary acute myeloid leukemia (AML) cells. Meisoindigo exhibits high antitumor activity[1][2].

体外研究
(In Vitro)

Meisoindigo (Dian III; 5-20 μM; for 24 hours) inhibits growth of the AML cell lines[1].
Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia[1].
Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest[1].
Meisoindigo (5-10 μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells[1].
Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells[2].

Cell Viability Assay[1]

Cell Line:HL-60, NB4, U937 leukemic cell lines
Concentration:5, 10, 15, 20 μM
Incubation Time:For 24 hours
Result:Inhibited growth of the AML cell lines in a dose- and time-dependent manner.

Apoptosis Analysis[1]

Cell Line:HL-60 cells
Concentration:10 μM
Incubation Time:For 24 hours
Result:Induced apoptosis of acute myeloid leukemia.

Cell Cycle Analysis[1]

Cell Line:HL-60 cells
Concentration:5, 10 μM
Incubation Time:For 24 hours
Result:Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line:HL-60 cells
Concentration:5, 10 μM
Incubation Time:For 24 hours
Result:Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells.
体内研究
(In Vivo)

Meisoindigo (Dian III; 50-150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo[1].
Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain[2].

Animal Model:NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cells[1]
Dosage:50, 100, 150 mg/kg
Administration:IP; daily; for 14 days
Result:Had anti-leukemic activity in vivo.
分子量

276.29

性状

Solid

Formula

C17H12N2O2

CAS 号

97207-47-1

中文名称

甲异靛

结构分类
  • Alkaloids
  • Indole Alkaloids
来源
  • Plants
  • other families
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 51 mg/mL(184.59 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.6194 mL18.0969 mL36.1939 mL
5 mM0.7239 mL3.6194 mL7.2388 mL
10 mM0.3619 mL1.8097 mL3.6194 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.05 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在本网站选购。