Biricodar

立即咨询

Biricodar

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	174254-13-8
包装:	10mg

包装与价格：

包装	价格(元)
10mg	电议

产品名称

VX-710, Incel

产品介绍

Biricodar (VX-710, Incel)是一种能够同时抑制P-gp和MRP活性的逆转剂，逆转活性很高，约为第一代抑制剂的100倍以上。

细胞实验
细胞系	HL60 cells line
方法	Cytotoxicity Assays. To study cytotoxicity in suspension cell lines, cells were plated in 96-well tissue culture plates at a density of 10,000 cells/well in RPMI 1640 supplemented with 10% FCS, 2 mm l-glutamine, 20 units/ml penicillin, and 20 μg/ml streptomycin. Drug was added to the culture medium to achieve final concentrations of 0.3 nm to 10 μm, with half-log increments, with and without VX-710 at a final concentration of 2.5 μM. The final volume of medium per well was 100 μl. Cells were incubated for 96 h at 37°C in a fully humidified atmosphere of 5% CO2 in air. Cell growth was assessed by the WST-1 colorimetric assay (Roche Diagnostics GmbH, Mannheim, Germany; Ref. 29 ), performed according to the manufacturer’s instructions. Briefly, 10 μl of WST-1 were added to each well, and culture plates were returned to the incubator for an additional 4 h. Subsequently, the absorbance at 450 nm (A450) and 600 nm (A600, background) was read for each well using a Microtek multiwell plate reader. Each drug exposure condition was assessed in quadruplicate.
浓度	2.5 μM
处理时间	96 h

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.6565 mL	8.2823 mL	16.5645 mL
5 mM	0.3313 mL	1.6565 mL	3.3129 mL
10 mM	0.1656 mL	0.8282 mL	1.6565 mL