Z-Asp-CH2-DCB

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Z-Asp-CH2-DCB

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

153088-73-4

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品介绍

Z-Asp-CH2-DCB 是一种不可逆的广谱caspase抑制剂，Z-Asp-CH2-DCB 也能抑制具有 caspase 样活性的蛋白酶。Z-D-CH2-DCB 并以剂量依赖的方式阻断 PBMC 细胞中的金黄色葡萄球菌肠毒素 B (SEB) 刺激的 IL-1β、TNF-α、IL-6 和 IFN-γ 的产生，和降低 SEB-1 刺激的 T 细胞增殖。Z-Asp-CH2-DCB 可预防 SU5416 诱导的间隔细胞凋亡和肺气肿的发生。

生物活性

Z-Asp-CH2-DCB is an irreversible broad spectrumcaspaseinhibitor. Z-Asp-CH2-DCB also inhibits proteases with caspase-like activity. Z-D-CH2-DCB blocks the production of IL-1β, TNF-α,IL-6, andIFN-γin staphylococcal enterotoxin B (SEB)-stimulated peripheral blood mononuclear cells (PBMC), and reduces SEB-1-stimulated T-cell proliferation in a dose-dependent manner. Z-Asp-CH2-DCB prevents SU5416-induced septal cellapoptosisand emphysema development^[1]^[2]^[3].

体外研究
(In Vitro)

Z-Asp-CH2-DCB (10-100 μM) blocks the production of IL-1β, TNF-α, IL-6, and IFN-γ in SEB-stimulated (200 ng; 16 hours) PBMC in a dose-dependent manner. The production of the chemokines MCP-1, MIP-1α, and MIP-1β was also suppresses. The inhibitory effect of Z-Asp-CH2-DCB on TSST-1-activated PBMC is similar, reducing IL-1β, IL-6, TNF-α, IFN-γ, MCP-1, MIP-1α, and MIP-1β to 10, 36, 25, 10, 11, 25, and 30%, respectively, of levels in untreated cells^[1].
Z-Asp-CH2-DCB (10-100 μM; 48 hours) inhibits T-cell proliferation in PBMC stimulated with 200 ng of SEB/ml^[1].

Cell Viability Assay^[1]

Cell Line:	Human peripheral blood mononuclear cells
Concentration:	10, 50, 100 μM
Incubation Time:	48 hours
Result:	Inhibited T-cell proliferation in PBMC stimulated with SEB.

体内研究
(In Vivo)

Z-Asp-CH2-DCB (1 mg; i.p.; every day for 3 weeks) prevents SU5416-induced septal cell apoptosis^[1].

Animal Model:	Male Sprague-Dawley rats (SU5416+ Z-Asp-CH2-DCB group)^[1]
Dosage:	1 mg
Administration:	Intraperitoneal injection; every day for 3 weeks
Result:	The caspase 3-like activity in SU5416-treated rat lungs is significantly higher, whereas lungs from rats treated with SU5416+Z-Asp-CH2-DCB showed no increase in apoptotic activity.

分子量

454.26

性状

Solid

Formula

C₂₀H₁₇Cl₂NO₇

CAS 号

153088-73-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(220.14 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.2014 mL	11.0069 mL	22.0138 mL
5 mM	0.4403 mL	2.2014 mL	4.4028 mL
10 mM	0.2201 mL	1.1007 mL	2.2014 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。