Adecatumumab (Anti-Human EPCAM Recombinant Antibody; MT201) 是 IgG1 同型的全人单克隆抗体，靶向人EpCAM。Adecatumumab 在几乎所有腺癌中都有表达，其活性与 K-Ras 状态无关。
| 生物活性 | Adecatumumab (Anti-Human EPCAM Recombinant Antibody; MT201) is a full human monoclonal antibody of theIgG1isotype, targeting humanEpCAM. Adecatumumab is expressed in almost all adenocarcinomas, and its activity is not dependent ofK-Rasstatus[1][2]. |
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体外研究
(In Vitro) | Adecatumumab (4 μM; 18 h) shows diverse kinetic binding activities among human Adecatumumab and murine Adecatumumab in B16/EpCAM 3E3 cells[2].
Adecatumumab (0.1 ng/mL-0.1 mg/mL; 4 h) shows a dose-dependent Antibodies depend on cell-mediated cytotoxicity (ADCC) activity in natural killing (NK) cells[2].
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体内研究
(In Vivo) | Adecatumumab (300 μg/mouse; i.v. bolus injection; 3 times per week) inhibits tumor growth in B16/EpCAM xenograft tumor model in mice, both of human adecatumumab and mu-adecatumumab[2].
Both human adecatumumab and mu-adecatumumab (300 μg/mouse; i.v. bolus injection; single dose) exhibit a bi-exponential curve progression of serum concentration with an early distribution phase between 0 and 10 h and a terminal elimination phase[2].
| Animal Model: | Female immunocompetent C57BL/6 mice (6-10 weeks old) with B16/EpCAM (i.v.)[2] | | Dosage: | 250 μg/mouse and 600 μg/mouse for human Adecatumumab; 125 μg/mouse and 300 μg/mouse for murine Adecatumumab | | Administration: | 250 μg/mouse and 600 μg/mouse for human Adecatumumab; 125 μg/mouse and 300 μg/mouse for murine Adecatumumab | | Result: | Both of them exhibited anti-tumor activity against B16/EpCAM cells in mice.
Although human adecatumumab inhibited the size of tumor colonies mice, the number of colonies was only slightly reduced after treatment without significant difference.
In contrast, mu-adecatumumab induced a highly significant reduction in the number of lung tumor colonies by >85%, and the few remaining tumor colonies were of very small size. |
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| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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