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D-glutamine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
D-glutamine图片
CAS NO:5959-95-5
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
D-谷氨酰胺
产品介绍
D-Glutamine 是可渗透细胞的谷氨酰胺 (Glutamine) 的 D 型立体异构体。
生物活性

D-Glutamine is a cell-permeable D type stereoisomer of Glutamine.

IC50& Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Glutamine is a key amino acid in the central nervous system (CNS), playing an important role in the glutamate/GABA-Glutamine cycle (GGC). In the GGC,Glutamine is transferred from astrocytes to neurons, where it will replenish the inhibitory and excitatory neurotransmitter pools[1]. D-Glutamine has been used to study its role in conferring protection against acetaldehyde-induced disruption of barrier function in Caco-2 cell monolayer. Role of L-Glutamine in the protection of intestinal epithelium from acetaldehyde-induced disruption of barrier function is evaluated in Caco-2 cell monolayer. L-Glutamine reduced the acetaldehyde-induced decrease in transepithelilal electrical resistance and increase in permeability to inulin and lipopolysaccharide in a time- and dose-dependent manner; D-Glutamine, L-aspargine, L-arginine, L-lysine, or L-alanine produced no significant protection. D-Glutamine also fails to influence the acetaldehyde-induced decrease in TER and increase in inulin flux. D-Glutamine or glutaminase inhibitor by themselves did not influence TER or inulin flux in control or acetaldehyde-treated cell monolayers. Lack of effect of D-Glutamine in protection from acetaldehyde indicates that the L-Glutamine-mediated protection is stereospecific[2].

分子量

146.14

性状

Solid

Formula

C5H10N2O3

CAS 号

5959-95-5

中文名称

D-谷氨酰胺

结构分类
  • Ketones, Aldehydes, Acids
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

H2O : 20 mg/mL(136.86 mM;ultrasonic and adjust pH to 2 with HCl)

DMSO : 4 mg/mL(27.37 mM;ultrasonic and warming and adjust pH to 5 with HCl and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM6.8428 mL34.2138 mL68.4275 mL
5 mM1.3686 mL6.8428 mL13.6855 mL
10 mM0.6843 mL3.4214 mL6.8428 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 12.5 mg/mL (85.53 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。