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Schisandrin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Schisandrin图片
CAS NO:7432-28-2
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
五味子醇甲
Schizandrin
Schizandrol
Schizandrol-A
产品介绍
Schisandrin (Schizandrin) 是一种从Schisandra chinensis的果实中分离出来的联苯环辛烯类木脂素。Schisandrin 具有抗氧化,保肝,抗癌和抗炎的活性。Schisandrin 还可以逆转大鼠的记忆障碍。
生物活性

Schisandrin (Schizandrin), a dibenzocyclooctadiene lignan, is isolated from the fruit ofSchisandra chinensisBaill. Schisandrin exhibits antioxidant, hepatoprotective, anti-cancer and anti-inflammatory activities. Schisandrin also can reverses memory impairment in rats[1][2][3].

体外研究
(In Vitro)

Schisandrin (10-100 μM; pretreated for 2 h) inhibits LPS-stimulated NO production, iNOS protein and mRNA expression in a dose-dependent manner in RAW 264.7 cells[1].
Schisandrin (25-100 μM; pretreated for 2 h) inhibits prostaglandin E2 production, COX-2 protein and mRNA expression in LPS-stimulated RAW 264.7 macrophages[1].
Schisandrin inhibits the SK-HEP-1, SNU-638, and T47D cells proliferation, with IC50s of 42.0, 53.1, and 40.0 μM, respectively[3].
Schisandrin (10-60 μM; 48 h) enhances Doxorubicin -induced apoptosis and selectively reverses MCF-7/DOX resistance[4].

Cell Viability Assay[1]

Cell Line:RAW 264.7 macrophages
Concentration:12.5, 25, 50, 100 μM
Incubation Time:Pretreated for 2 h and then incubated with LPS (1 μg/mL) for 18 h
Result:Markedly decreased iNOS protein expression a dose-dependent manner.
Significantly inhibited COX-2 protein expression.
体内研究
(In Vivo)

Schisandrin (10-100 mg/kg; a single i.p.) inhibits acetic acid-induced peritoneal vascular permeability and reduces the carrageenan-induced paw edema of mice[1].
Schisandrin (1-10 mg/kg; a single p.o.) significantly reverses the Scopolamine-induced impairment of spatial memory and passive avoidance response, and enhances tremors induced by Oxotremorine in rats[2].

Animal Model:Male ICR mice (4-weeks) were treated with λ-carrageenan by intraperitoneal injection into the right hind paw[1]
Dosage:100, 200 mg/kg
Administration:A single i.p.
Result:Inhibited paw edema by 33.43 % (100 mg/kg) and 57.38 % (200 mg/kg) at 3 h.
分子量

432.51

性状

Solid

Formula

C24H32O7

CAS 号

7432-28-2

中文名称

五味子素;五味子醇甲

结构分类
  • Phenylpropanoids
  • Lignans
来源
  • Plants
  • Schisandraceae
  • Schisandra chinensis(Turcz.) Baill.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 1 mg/mL(2.31 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.3121 mL11.5604 mL23.1209 mL
5 mM---------
10 mM---------
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 0.28 mg/mL (0.65 mM); Clear solution

    此方案可获得 ≥ 0.28 mg/mL (0.65 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 2.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在本网站选购。