| 生物活性 | Ginkgetin, a biflavone, is isolated from Ginkgo biloba leaves. Ginkgetin exhibit anti-tumor, anti-inflammatory, neuroprotective, anti-fungal activities. Ginkgetin is also a potent inhibitor ofWntsignaling, with anIC50of 5.92 μΜ[1][2][3][4][5]. |
体外研究
(In Vitro) | Ginkgetin (2.5-20 μM; 48 h) inhibits the growth of Daoy and D283 cell lines, and induces G2/M cell cycle arrest in Daoy cells[2].
Ginkgetin (20-40 μM; 24 h) significantly activates the apoptosis of osteosarcoma cells in a concentration-dependent manner[3].
Ginkgetin (10-20 μM; 3-24 h) down-regulated the expression of Wnt target genes without affecting the expression of β-catenin in medulloblastoma cells[2].
Ginkgetin (1-10 μM; 24 or 48 h) significantly inhibits the VEGF-induced endothelial cell proliferation, migration, and wound recovery in a concentration-dependent manner[1].
Ginkgetin (5-10 μM; 48 h) induces autophagy responsible for cell death in A549[5].
Cell Viability Assay[2] | Cell Line: | Daoy and D283 cell lines | | Concentration: | 2.5, 5, 10, 20 μM | | Incubation Time: | 48 hours | | Result: | Inhibited the cell growth, with IC50s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively. |
Apoptosis Analysis[3] | Cell Line: | Osteosarcoma cells | | Concentration: | 20, 30, 40 μM | | Incubation Time: | 24 hours | | Result: | Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner. |
Cell Cycle Analysis[2] | Cell Line: | Daoy cells | | Concentration: | 2.5, 5, 10, 20 μM | | Incubation Time: | 24 hours | | Result: | Increased G2/M phase, compared with that of control, indicating a G2/M cell phase arrest. |
Cell Cycle Analysis[2] | Cell Line: | Daoy and D283 cell lines | | Concentration: | 10, 20 μM | | Incubation Time: | 3, 6, 12, 24 hours | | Result: | Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells.
Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner. |
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体内研究
(In Vivo) | Ginkgetin (25-100 mg/kg; i.p. 2 hours after the onset of ischemia) exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway[4].
Ginkgetin (30 mg/kg; intragastrically once per day for 42 d) suppresses tumor growth in A549 cells bearing nude mice[5].
| Animal Model: | Male Sprague-Dawley rats (200-220 g)[4] | | Dosage: | 25, 50, 100 mg/kg | | Administration: | I.p. 2 hours after the onset of ischemia | | Result: | Reduced the neurological deficit score.
Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα.
Decreased the expressions of ICAM-1, COX-2, and iNOS.
Downregulated downstream inflammatory factor PGE2 and TNF-α expression.
Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression. |
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| 来源 | - Plants
- Ginkgoaceae
- Ginkgo biloba
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 20.83 mg/mL(36.77 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 1.7652 mL | 8.8260 mL | 17.6519 mL | | 5 mM | 0.3530 mL | 1.7652 mL | 3.5304 mL | | 10 mM | 0.1765 mL | 0.8826 mL | 1.7652 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 0.5%CMC-Na/saline water Solubility: 2 mg/mL (3.53 mM); Suspended solution; Need ultrasonic and warming and heat to 60℃ 2. 请依序添加每种溶剂： 50%PEG300 50% saline Solubility: 2 mg/mL (3.53 mM); Suspended solution; Need ultrasonic and warming
*以上所有助溶剂都可在本网站选购。 |
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