Phellamurin 是一种来自Phellodendron amurense叶的植物黄酮糖苷，可抑制肠道 P-糖蛋白 (P-glycoprotein)。Phellamurin 还抑制凤蝶的产卵。Phellamurin 诱导细胞凋亡 (apoptosis)，并具有抗肿瘤活性。
| 生物活性 | Phellamurin is a plant flavonone glycoside from the leaves ofPhellodendron amurenseand inhibits intestinalP-glycoprotein. Phellamurin also inhibits egg laying by Papilio protenor. Phellamurin induces cellsapoptosisand has anti-tumor activity[1][2][3]. |
体外研究
(In Vitro) | Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment leads to a repression of cell viability in U2OS and Saos-2 cells in a dose-dependent manner[1].
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment concentration-dependently promots the apoptosis of U2OS and Saos-2 cells[1].
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment declines the ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR in U2OS and Saos-2 cells[1].
Cell Viability Assay[1] | Cell Line: | U2OS and Saos-2 cells | | Concentration: | 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL | | Incubation Time: | 48 hours | | Result: | Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner. |
Apoptosis Analysis[1] | Cell Line: | U2OS and Saos-2 cells | | Concentration: | 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL | | Incubation Time: | 48 hours | | Result: | Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner. |
Western Blot Analysis[1] | Cell Line: | U2OS and Saos-2 cells | | Concentration: | 0 μg/mL and 10 μg/mL | | Incubation Time: | 48 hours | | Result: | Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells. |
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体内研究
(In Vivo) | Phellamurin (50 mg/kg/day; intraperitoneal injection; daily; for 21 days; female BALB/c nude mice) treatment represses osteosarcoma tumor growth in vivo. The ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR are decreased in xenograft in Phellamurin-treated mice[1].
| Animal Model: | Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells[1] | | Dosage: | 50 mg/kg/day | | Administration: | Intraperitoneal injection; daily; for 21 days | | Result: | Repressed osteosarcoma tumor growth in vivo. |
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| 来源 | - Plants
- Rutaceae
- Phellodendron amurenseRupr.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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