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Laminaran
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Laminaran图片
CAS NO:9008-22-4
包装与价格:
包装价格(元)
100mg电议
200mg电议
500mg电议

产品名称
昆布多糖
产品介绍
Laminaran 是一种β-1-3-葡聚糖,源于Eisenia Bicyclis,Dectin-1 的典型配体,具有免疫调节,辐射防护作用以及抗癌活性。 Laminaran 由具有 β (1→6) 分支的 β (1→3)-葡聚糖组成,可以被葡糖酶诸如 (EC 3.2.1.6) 之类的酶催化,该酶会破坏 β (1→3) 键。 Laminaran 是一种有前途的免疫刺激分子,用于癌症免疫的研究。
生物活性

Laminaran is anβ-1-3-glucanand a typical ligand forDectin-1fromEisenia Bicyclis, has potent immunomodulating, radioprotective, and anticancer activities[1]. Laminaran is made up of β (1→3)-glucan with β (1→6)-branches and can be catalyzed by enzymes such as laminarinase (EC 3.2.1.6) that breaks the β (1→3) bonds[2]. Laminaran is a promisingimmune stimulatory moleculefor use incancer immunotherapy[3].

体外研究
(In Vitro)

Laminaran (100-800 μg/mL; 24 hours) is not cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28, the percentage of inhibition of living cells number is less than 15 % at concentrations range up to 800 μg/mL after 24 h of treatment[1].
Laminaran (200 μg/mL; 24-72 hours)does not cause any growth inhibition of SK-MEL-28 cells after 24 and 48 h of treatment, but decreases cells proliferation by 36 % after 72 h of treatment[1].
Laminaran (25-50 μg/mL; 24 hours) at low concentration does not influence the phosphorylation of c-Raf (Ser259), ERK1/2 (Tyr202/Tyr204), and MEK1/2 (Ser 221) kinases as well as total expression level of investigated proteins. But decreases p-MEK, p-ERK1/2 at 50 μg/mL[1].

Cell Viability Assay[1]

Cell Line:JB6 Cl41 and SK-MEL-28 cells
Concentration:100, 200, 400, and 800 μg/mL
Incubation Time:24 hours
Result:Showed no cytotoxic against normal epidermal cells JB6 Cl41 and human melanoma cells SK-MEL-28.

Cell Proliferation Assay[1]

Cell Line:SK-MEL-28 cells
Concentration:200 μg/mL
Incubation Time:24 hours
Result:Decreased cell proliferation at 72 hours.

Western Blot Analysis[1]

Cell Line:SK-MEL-28 cells
Concentration:25 μg/mL; 50 μg/mL
Incubation Time:24 hours
Result:Inhibited phosphorylation of c-Raf, MEK1/2, and ERK1/2 kinases.
体内研究
(In Vivo)

Laminaran (intravenous injection; 12.5, 25, and 50 mg/kg; 21 days) and OVA (50 μg) combination significanly decreases the tumor masses when it compares with the PBS-, OVA-, and laminarin-treated mice[3].

Animal Model:C57BL/6 mice injected s.c. with B16-OVA cells[3]
Dosage:12.5, 25, and 50 mg/kg; 21 days
Administration:Intravenous injection
Result:Prevented B16-OVA tumor growth by inducing Ag-specific immune responses.
性状

Solid

Formula

C18H32O16

CAS 号

9008-22-4

中文名称

昆布多糖;褐藻淀粉

结构分类
  • Saccharides
  • Polysaccharides
来源
  • Plants
  • other families

Eisenia Bicyclis

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL(Need ultrasonic)

H2O : 16.67 mg/mL(Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 33.33 mg/mL (Infinity mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (Infinity mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (Infinity mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。