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Mavorixafor(AMD-070)triHCl
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Mavorixafor(AMD-070)triHCl图片
CAS NO:880549-30-4
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW) 458.86
Formula C21H30Cl3N5
CAS No.880549-30-4 (HCl)
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO:>10 mM
Water: NA
Ethanol: NA
Chemical Name (S)-N1-((1H-benzo[d]imidazol-2-yl)methyl)-N1-(5,6,7,8-tetrahydroquinolin-8-yl)butane-1,4-diamine trihydrochloride
Synonyms X4P-001 3HCl; AMD 11070 3HCl; AMD-070 3HCl; X4P 0013HCl; AMD-11070 3HCl; AMD 070 3HCl; X4P001 3HCl; AMD11070 3HCl; AMD070 3HCl; mavorixafor 3HCl
SMILES Code NCCCCN(CC1=NC2=CC=CC=C2N1)[C@H]3CCCC4=C3N=CC=C4.[H]Cl.[H]Cl.[H]Cl
实验参考方法
In Vitro

In vitro activity: AMD-070 is active against X4 strain of HIV-1, HIV-1 IIIb in MT-4 cells, and The IC50 values for AMD-070 are 9-fold higher (0.009 μM vs 0.001 μM) and 8.7-fold higher (0.003 μM vs 0.026 μM) in PBMCs compared to MT-4 cells. AMD-070 has antiviral ability with the IC50 value of 15.5 nM.


Kinase Assay: SUP-T1 T cells are first preincubated with the compounds (with 1 as a control) for 30 min on ice, washed with PBS with 2% FCS, and incubated with PE-conjugated anti-CXCR4 mAb for 30 min on ice. After being washed with PBS, the cell samples are fixed with 1% paraformaldehyde in PBS and analyzed on a FACS Calibur flow cytometery. The dose-dependent inhibitory effects of the compounds on mAb binding are determined using the mean fluorescence intensity values.


Cell Assay: he activated cells (PHA-stimulated blasts) are washed three times with PBS, and viral infections are done. HIV-infected or mock-infected PHA-stimulated blasts are cultured in the presence of 25 U/mL of IL-2 and varying concentrations of compounds. Supernatant is collected at day 10, and HIV-1 core antigen in the culture supernatant is analyzed by the p24 viral Ag ELISA kit. Inhibition of HIV-1 replication in MT-4 cells is performed as previously described. Anti-HIV-1 activity and cytotoxicity measurements are carried out in parallel. They are based on the viability of MT-4 cells that has been infected with HIV-1 in the presence of various concentrations of the test compounds. The ICsub>50 is defined as the concentration required to inhibit 50% of the virus-infected cells against viral cytopathicity.

In Vivo AMD-070 shows promising oral bioavailability in rat and dog. The rate of clearance is species dependent with AMD-070 having lower clearance in dog compared to rat.
Animal model Rat
Formulation & Dosage 2.5 mg/kg, i.v.
References J Med Chem. 2010 Apr 22;53(8):3376-88.